UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells

Figure 6

UFL1 modulates the TLR4/NF-κB signaling pathway in LPS-stimulated BMECs. (a) The effects of si-TLR4 transfection on TLR4 and NF-κB P65 expression. (b) Representative immunoblots and quantification for analysis of TLR4, IκBα, and phospho-IκBα in UFL1 siRNA-transfected BMECs stimulated with LPS. (c) Representative immunoblots and quantification for analysis of TLR4, IκBα, and phospho-IκBα in BMECs transfected with UFL1 overexpression plasmid. (d) The expression of p65 and p-p65 in the cytoplasm and nucleus of UFL1 siRNA-transfected BMECs stimulated with LPS. (e) The expression of p65 and p-p65 in the cytoplasm and nucleus of BMECs transfected with UFL1 overexpression plasmid. (f) Immunofluorescent staining of BMECs with anti-NF-κB antibody in siRNA-treated cells stimulated with LPS. (g) Immunofluorescent staining of BMECs with anti-NF-κB antibody in UFL1 overexpression plasmid-treated cells stimulated with LPS. Data are presented as the of the mean (SEM) of three independent experiments. ; .

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