HDAC6 Inhibition Protects against OGDR-Induced Golgi Fragmentation and Apoptosis

Figure 1

Fragmentation of GA and tubulin acetylation in N2a cells after OGDR. The experiment was repeated independently for at least three times. (a) Immunofluorescent stain using antibodies against acetylated tubulin (red color) and Golgi marker TGN38 (green color) and counterstain with 4,6-diamidino-2-phenylindole (blue color) to show nuclei revealed normal GA in normal conditions. However, more and more N2a cells showed fragmented GA after 4 h reperfusion following 4 h OGD. (b) Quantitation () of (a) from three independent experiments. The proportion of N2a cells with fragmented GA increased after 4 h reperfusion following 4 h OGD exposure. (c) Quantitation () of (a) from three independent experiments. Tubulin acetylation was significantly downregulated after 4 h reperfusion following 4 h OGD in N2a cells. The western blot data also showed that OGDR insult decreased tubulin acetylation in N2a cells. and compared to normal conditions. .

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