HDAC6 Inhibition Protects against OGDR-Induced Golgi Fragmentation and Apoptosis
Effect of HDAC6 inhibition on GA morphology in cultured neurons and N2a cells exposed to OGDR. Cultured neurons and N2a cells were transfected with different shRNA. After transfection for 36 h, cultured neurons and N2a cells were treated with 4 h OGD plus 2 h reperfusion or 4 h reperfusion, respectively. The experiment was repeated independently for at least three times. (a) Digital photomicrograph under fluorescent illumination showing the morphology of GA which was detected using anti-TGN38 staining. Fragmented GA was evident in cultured neurons transfected with control shRNA exposed to 4 h OGD plus 2 h reperfusion. Transfection with HDAC6 shRNA significantly attenuated OGDR-induced fragmentation of GA. (b) Transfection with HDAC6 shRNA also significantly attenuated OGDR-induced fragmentation of GA in N2a cells. (c) Quantitation () of (a) from three independent experiments. (d) Quantitation () of (b) from three independent experiments. (e) Pretreatment with tubacin significantly ameliorated OGDR-induced GA fragmentation in N2a cells. Values are expressed as . compared to control. .
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