HDAC6 Inhibition Protects against OGDR-Induced Golgi Fragmentation and Apoptosis

Figure 6

Inhibition of HDAC6 confers protection against OGDR-induced apoptosis in N2a cells. (a) Mouse N2a cells transfected with HDAC6 shRNA or control shRNA were subjected to 4 h OGD plus 4 h reperfusion, and western blot was performed to examine the protein levels of acetylated tubulin, activated caspase 3, p115 cleavage, and HDAC6. Inhibition of HDAC6 contributed to increase tubulin acetylation and decrease caspase 3 activation and p115 cleavage. (b) Quantitation () of (a) from three independent experiments. The expression of HDAC6 is downregulated after HDAC6 shRNA transfection. HDAC6 shRNA transfection also attenuated OGDR-induced tubulin deacetylation, caspase 3 activation, and p115 cleavage. (c) Downregulation of HDAC6 was able to suppress OGDR-induced apoptosis. (d) Quantitation () of (c) from three independent experiments. (e) Pretreatment with tubacin significantly ameliorated OGDR-induced apoptosis in N2a cells. and compared to control.

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