Triptolide Inhibits Preformed Fibril-Induced Microglial Activation by Targeting the MicroRNA155-5p/SHIP1 Pathway

Figure 1

αSyn activates primary microglial aggregation-dependently. (a) Schematic overview of the preparation of different αSyn species. (b) Size exclusion chromatography HLPC (SEC-HLPC) was performed to determine the HNE-mediated oligomerization after being incubated at 37°C for 24 h. (c) Thioflavin T was used to confirm the beta sheet conformation that characterizes the amyloid-like PFFs after agitation for 7 days at 37°C. Levels of TNFα (d) and IL-1β (e) were measured after exposing to monomers, oligomers, or PFFs for 24 h in primary microglia. The values were expressed as . , , and .

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