Deuterium Incorporation Protects Cells from Oxidative Damage

Figure 5

Effect of deuterated nucleoside incorporation on the extent of oxidative DNA damage in Jurkat cells. (a–c) The effect of increasing concentrations of D-dA, D-dG, and D-dT, respectively, on the extent of DNA single-strand breakage caused by a 30 min challenge with 50 μM H₂O₂. (d–f) The effect of a fixed dose of D-dA (500 μM), D-dG (50 μM), and D-dT (100 μM), respectively, on the DNA damage induced by increasing concentrations of H₂O₂. NDF (nuclear diffusion factor) represents the extent of DNA damage measured by FHA. We show the results as of at least five experiments. , , and (one-way ANOVA).

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