The Effect of Maternal Diet with Fish Oil on Oxidative Stress and Inflammatory Response in Sow and New-Born Piglets

Figure 3

Effect of maternal diet with fish oil on anti-inflammatory parameters in placentas and livers of piglets. PGE2 concentrations (a) in plasma collected from sows on the 84th day of gestation (G84d) and farrowing day (Fd), cord blood (CB), and new-born piglets (NBP) were determined by the ELISA method. Relative expression levels of TAB1, TAK1, PTGS2, Alox5, GPR120, TLR4, NF-κB, and PPARγ mRNA in the placentas and livers of new-born piglets collected from sows fed different diets (b) were determined by real-time PCR. The protein expression of JNK, p-JNK, p38α, p-p38, ERK1/2, phospho-ERK1/2, IκBα, and p-IκBα in the placentas (c) and livers (d) of new-born piglets collected from sows fed different diets was determined by Western blot analysis. The values are calculated as the ratios of phosphorylation levels (p-JNK, p-p38, p-ERK1/2, and p-IκB) to the total levels of JNK, p38, ERK1/2, and IκB. Values are means (), with their standard errors represented by vertical bars. Mean values were significantly different between the two diet groups (). SD=soybean oil diet; FD=fish oil diet. Note: p38 (p38α (C20), SC-535, Santa Cruz Biotechnology Inc.) is recommended for the detection of p38α as the total protein, while p-p38 (p-p38 (D-8) SC7379, Santa Cruz Biotechnology Inc.) is recommended for the detection of p38α, p38β, and p38γ correspondingly.

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