Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells

Figure 5

The frequency of early and late apoptotic cells in LOVO/DX cell cultures incubated with celastrol (a) and coincubated with celastrol and NAC (b). The cells were double stained with Annexin V-FITC and PI fluorescent dyes (FITC Annexin V Apoptosis Detection Kit) and analyzed by flow cytometry. Control: LOVO/DX cells incubated without celastrol (gray), LOVO/DX cells incubated without celastrol and NAC (black). The results are presented as a percentage of early apoptotic cells (Annexin V-FITC⁺ and PI^-) and late apoptotic cells (Annexin V-FITC⁺ and PI⁺); , , , , and .

(a)

(b)