Increased Oxidative Stress Induced by Rubus Bioactive Compounds Induce Apoptotic Cell Death in Human Breast Cancer Cells
Effect of C1 and C2 on cellular viability, proliferation, and cytotoxicity. (a) Trypan blue viability. Trypan blue viability test showed a significant () decrease in the viability of the MCF-7 cells after C1 and C2 treatment compared to the control cells. (b) The ATP proliferation assay. ATP luminescent cell assay was used to determine MCF-7 cell proliferation after the treatment with C1 and C2. Control cells showed an increased ATP level, whereas a dose-dependent significant ( and ) decrease in ATP level was observed in both experimental groups. (c) The lactate dehydrogenase (LDH) cytotoxicity. The LDH cytotoxicity test showed a significant increase in cytotoxicity of cells after the 24 h treatment with C1 and C2 compared to control cells. The significant differences between the treated and control groups are shown as and .
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