Physical Exercise Attenuates Oxidative Stress and Morphofunctional Cerebellar Damages Induced by the Ethanol Binge Drinking Paradigm from Adolescence to Adulthood in Rats

Lamarão-Vieira, Kátia; Pamplona-Santos, Dinair; Nascimento, Priscila C.; Corrêa, Márcio G.; Bittencourt, Leonardo O.; dos Santos, Savio M.; Cartágenes, Sabrina C.; Fernandes, Luanna Melo Pereira; Monteiro, Marta C.; Maia, Cristiane S. F.; Lima, Rafael R.

doi:https://doi.org/10.1155/2019/6802424

Oxidative Medicine and Cellular Longevity

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Research Article | Open Access

Volume 2019 | Article ID 6802424 | https://doi.org/10.1155/2019/6802424

Physical Exercise Attenuates Oxidative Stress and Morphofunctional Cerebellar Damages Induced by the Ethanol Binge Drinking Paradigm from Adolescence to Adulthood in Rats

Kátia Lamarão-Vieira,¹Dinair Pamplona-Santos,¹Priscila C. Nascimento,¹Márcio G. Corrêa,¹Leonardo O. Bittencourt,¹Savio M. dos Santos,²Sabrina C. Cartágenes,³Luanna Melo Pereira Fernandes,³Marta C. Monteiro,²Cristiane S. F. Maia,³and Rafael R. Lima¹

Academic Editor: Jeferson L. Franco

Received27 Aug 2018

Accepted06 Nov 2018

Published18 Feb 2019

Abstract

Ethanol (EtOH) binge drinking is characterized by high EtOH intake during few hours followed by withdrawal. Protection strategies against the damages generated by this binge are poorly explored. Thus, this study is aimed at investigating the protective role of treadmill physical exercise (PE) on the damage caused after repeated cycles of binge-like EtOH exposure in the oxidative biochemistry, morphology, and cerebellar function of rats from adolescence to adulthood. For this, animals were divided into four groups: control group (sedentary animals with doses of distilled water), exercised group (exercised animals with doses of distilled water), EtOH group (sedentary animals with doses of 3 g/kg/day of EtOH, 20% w/v), and exercised+EtOH group (exercised animals with previous mentioned doses of EtOH). The PE occurred on a running treadmill for 5 days a week for 4 weeks, and all doses of EtOH were administered through intragastric gavage in four repeated cycles of EtOH in a binge-like manner. After the EtOH protocol and PE, animals were submitted to open field and beam walking tests. In sequence, the cerebellums were collected for the biochemical and morphological analyses. Biochemical changes were analyzed by measurement of Trolox equivalent antioxidant capacity (TEAC), reduced glutathione content measurements (GSH), and measurement of nitrite and lipid peroxidation (LPO). In morphological analyses, Purkinje cell density evaluation and immunohistochemistry evaluation were measured by antimyelin basic protein (MBP) and antisynaptophysin (SYP). The present findings demonstrate that the binge drinking protocol induced oxidative biochemistry misbalance, from the decrease of TEAC levels and higher LPO related to tissue damage and motor impairment. In addition, we have shown for the first time that treadmill physical exercise reduced tissue and functional alterations displayed by EtOH exposure.

1. Introduction

Ethanol (EtOH) is a global public health concern. Its abusive consumption involves about 3.3 million of deaths per year (5.9% of all world deaths) and 5.1% of morbidity in the world [1]. From the pharmacological point of view, EtOH is a dose-dependent psychotropic drug that when consumed in large proportions increases the risk of morbidity and/or mortality [2, 3].

Over the years, the pattern of EtOH consumption has shown significant changes, both in quantity and frequency [4]. Binge drinking has been considered a toxic and dangerous practice because it is an intense consumption (≤0.08 g/dL of EtOH in the blood) in a single session, followed by abstinence, which causes changes in the central nervous system (CNS) [5, 6]. Nowadays, the most emerging pattern of EtOH intake by adolescents and young people is the binge drinking, characterized by high EtOH intake during few hours followed by withdrawal [6, 7].

In face of the high prevalence of EtOH consumption, investigating modifiable factors for CNS damage becomes an important aim for neuroprotective strategies. Physical exercise (PE) has been shown to modulate neural functions due to its neuronal loss preventions after several brain damages. Also, it acts on behavioral improvement and structural alteration in the brain [8], promotes plasticity [9–11], modulates the oxidative biochemistry and synaptic transmission [12, 13], increases brain-derived neurotrophic factors (BDNF), and stimulates angiogenic processes [10].

Considering the epidemiological relevance of EtOH and the possible injuries to the cerebellum, organ that plays an important role in the control of the sensorimotor system, motor coordination, and motor cognition [14–16], this study is aimed at investigating the action of moderate treadmill PE as a possible neuroprotective strategy to avoid damages displayed by the EtOH binge drinking pattern over the cerebellum, as well as the motor functions associated to this brain structure of rats.

2. Materials and Methods

2.1. Animals and Experimental Design

Forty male Wistar rats, weighing 100-150 g with the age of 30 days, were used in this investigation. The animals were maintained in collective cages (4 animals each) with water and food ad libitum and kept in a climate-controlled room (25°C) with a 12 : 12 h light/dark cycle (lights on 7:00 AM). All procedures were previously approved by ethics committee on animal experimentation by Federal University of Pará, under the protocol CEPAE-UFPA: 227-14, and following the NIH Guide for the Care and Use of Laboratory Animals [17].

Experimental animals were randomly distributed into four groups: control group (sedentary animals that received distilled water by gavage, ), exercised group (exercised animals that received distilled water by gavage, ), EtOH group (sedentary animals that received 3 g/kg/day of EtOH, 20% (w/v) by gavage, ), and exercised+EtOH group (exercised animals with EtOH doses previously mentioned, ). The experimental design is summarized in Figure 1.

Figure 1

Sample description and experimental stages. (a) Description of the sample and division of experimental groups of treadmill physical exercise and ethanol (EtOH) or distilled water (H₂Od) administration. (b) The behavioral assays: open field and beam walking tests. (c) Cerebellum collection for analyses. (d) Oxidative balance analyses through Trolox equivalent antioxidant capacity (TEAC), reduced glutathione (GSH), lipid peroxidation (LPO), and nitrite levels (nitrite). (e) Histological analysis by quantification of Purkinje cells (PC) and immunohistochemically evaluation through anti-synaptophysin (SYP) and anti-myelin basic protein (MBP).

The PE protocol was adapted from Arida et al. [18], in which the animals were subjected to 5 consecutive training days per week for 4 weeks on the treadmill without inclination degree (MasterOne). The physical exercise scale is described in Figure 2. Five days after treadmill PE, one cycle of binge-like EtOH treatment was performed. This cycle involves a single EtOH administration per day by oral gavage, for 3 consecutive days (3 days followed by 4 days off). Control subjects received only distilled water, in the same paradigm [5, 19, 20]. The cycle of treadmill PE and binge-like EtOH exposure lasted for 4 weeks, and the animals were weekly weighted for dose adjustment.

2.2. Behavioral Assay

Twenty-four hours after the last protocol day of EtOH intake and treadmill PE, animals were conducted to the assay room for motor behavioral tests. Open field and beam walking tests were conducted between 11:00 AM and 6:00 PM in a sound-attenuated room under low-intensity light (12 lux), where the rats had been habituated for at least 2 h before beginning the tests.

2.2.1. Open Field Test

The analyses of spontaneous locomotion and vertical exploratory activity were assessed by open field protocol [21]. Animals were placed on the arena (), wherein the floor is divided into 25 equal quadrants (). Firstly, each animal is placed in the center of the floor and observed for five minutes. The number of total intersections was analyzed by the ANY-maze™ software (Stoelting, USA), and the number of standing position (rearing) was analyzed manually.

2.2.2. Beam Walking Test

After open field assay, animals were submitted to beam walking test. In this experiment, motor coordination and balance were assessed based on animals’ ability to traverse a graded series of narrow beams to reach an enclosed safety platform. The apparatus consists of wooden beams (100 cm length) suspended 50 cm from the floor, which allows the rat to access a secure platform (closed box of ). Briefly, animals were acclimated in the beams of a higher cross-sectional area, and a limit of 120 s was fixed to reach the box. In order to intensify the task difficulty, animals were submitted to two test sessions (cut-off 60 s each) on each beam of square cross section (12 mm and 5 mm), respectively. The latency to reach the closed box was measured in seconds, and the number of foot slips was registered considering one or both hind limbs slipped from the beam [22, 23].

After behavioral tests, five animals per group were euthanized by cervical dislocation and the cerebellums were immediately removed for biochemical assay. The other 5 animals from each group were perfused, and the cerebellums were destined for histological evaluations.

2.3. Oxidative Biochemistry Analyses

After cerebellum collection, the dissected tissue was cleaned in saline solution, frozen in liquid nitrogen, and subsequently stored at −80°C. Then, the tissue was thawed and resuspended in Tris-HCl 20 mM, pH 7.4 at 4°C, and sonically disaggregated. The preparation of the tissue for oxidative biochemistry analysis has been described in detail in our previous studies [22, 24].

2.3.1. Measurement of Trolox Equivalent Antioxidant Capacity (TEAC)

For TEAC level analyses, we used the method described by Rufino et al. [25] and the method adapted from our previous study [26]. Briefly, the 2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS; 7 mM) was incubated by adding potassium persulfate (2.45 mM) at room temperature during 16 h to produce ABTS+ radical. The work solution was prepared from ABTS+ radical in phosphate basic saline (PBS) solution (pH 7.2) until absorbance of at 734 nm. Subsequently, 35 μL of this solution or Trolox standards (standard curve) was added to 2970 μL of ABTS solution, and absorbance was acquired after 5 minutes. Absorbances were measured in triplicate and calculated following a standard curve with Trolox [27] standard concentrations. Total antioxidant capacity was expressed in μmol/L.

2.3.2. Reduced Glutathione Content Measurements (GSH)

Determination of the GSH levels was based on the ability of GSH to reduce 5,5-dithiobis-2-nitrobenzoic acid (DTNB) to nitrobenzoic acid (TNB), which was quantified by spectrophotometry at 412 nm. Thus, the methodology described by Ellman [28] was adapted for this determination. Initially, an aliquot (20 μL) from supernatant was added in a tube containing distilled water (20 μL) and PBS solution pH 8.0 (3 mL) to carry out the first measurement. Afterwards, 5,5-dithiobis (2-nitrobenzoic acid) (DTNB; 0.47mMol) was added to the solution, and another measurement was carried out after 3 minutes [26, 29]. The GSH concentration was expressed as μg/mL.

2.3.3. Determination of Lipid Peroxidation (LPO)

Lipid peroxidation was estimated as the levels of malondialdehyde (MDA) and 4-hydroxyalkenes (4HDA) as earlier detailed by Esterbauer and Cheeseman [30]. As previously described in our studies [5, 31], an aliquot of the supernatant was processed as described by the Bioxytech LPO-568 kit (Cayman Chemical). This kit is a chromogenic reagent that reacts with MDA at 45°C. The absorbance measurement on spectrophotometers was performed at 586 m wavelength.

2.3.4. Nitrite Level Quantification

For quantification of nitrite, we used the protocol described by Green et al. [32], which consists of using an aliquot of crude homogenate that was centrifuged at 21,000 for 20 min at 4°C and the supernatant to analyze nitrite levels. Briefly, the samples were incubated at room temperature for 20 min with the Griess reagent (0.1% naphthylethylenediamine and 1% sulfanilamide in 5% phosphoric acid—1 : 1). The absorbance was measured at 550 nm by a spectrophotometer and compared to the standard sodium nitrite solutions.

2.3.5. Protein Concentration Assay

The measurement of protein content in the supernatants (20 μL) was performed as described by Bradford [33] in order to correct the values of LPO and nitrite by protein concentration. Thus, results of LPO and nitrite levels were expressed in percentages of control groups.

2.4. Histological Evaluation

After behavioral assessment, 5 animals of each group were used for tissue analysis. The animals were deeply anesthetized with ketamine hydrochloride (90 mg/kg) and xylazine hydrochloride (10 mg/kg) solution and transcardially perfused with heparinized 0.1 M phosphate-buffered saline (PBS) followed by 4% paraformaldehyde. The cerebellums were removed from the cranial cavity and postfixed for 4 hours in Bouin solution. Then, the cerebellums were dehydrated in increasing EtOH solutions, diaphanized in xylol, and embedded in paraplast (McCormick®). Sections of 5 μm thickness were obtained by coronal cuts in microtome and then put on microscopy slides.

For analysis of Purkinje cells, tissue sections were stained with hematoxylin and eosin (HE) and for immunohistochemistry analysis the sections of the same animal cerebellum were mounted on 3-aminopropyltriethoxysilane-coated (Sigma®) microscopy slides. The number of Purkinje cells was evaluated by using a square 0.25 mm wide grid in the eyepiece of the microscope for quantitative assessments. This grid corresponds to an area of 0.0625 mm². At least 3 fields in the cerebellum per section and 3 sections per animal of each group were analyzed, adapted from Lima et al. [34] for the cerebellum.

2.4.1. Immunohistochemistry

Myelin impairment was evaluated using an antibody against rat myelin basic protein (MBP), an important component of the compact myelin. In addition, we used antisynaptophysin (SYP) for immunostaining of neurosecretory vesicles.

All immunohistochemistry procedures were conducted according to the previously published studies [34–36]. Briefly, sections were dewaxed in xylene, hydrated in decreasing ethanol solutions (absolute 2, absolute 1, 90%, 80%, 70%), and rinsed in 0.1 M PBS for 5 min. Antigen recovery was performed with citrate buffer solution (pH 6.0), previously heated to 60°C, for 20 min. After that, sections were further allowed to cool for about 20 min and incubated in 1% hydrogen peroxide solution (H₂O₂) in methanol for 20 min for the inhibition of endogenous peroxidase activity. Then, sections were rinsed three times in 0.1 M PBS/Tween (Sigma®) solution for 5 min and incubated with 10% normal horse serum and 3% bovine serum albumin (BSA, Sigma®) in PBS for 1 h. Without further rinsing, sections were then incubated overnight with the primary antibody in PBS: anti-MBP (1 : 100, Chemicon®) and anti-MBP (1 : 100, Serotec®), rinsed in PBS/Tween solution for 5 min (three times), and incubated with biotinylated horse anti-mouse (1 : 100) secondary antibody (Vector Laboratories®), for 2 h. Sections were rinsed again for 5 min (three times) and incubated in avidin-biotin-peroxidase complex (ABC Kit, Vector Laboratories®) for 2 h. Sections were rinsed three times (five minutes each) in 0.1 M phosphate-buffered saline and revealed with 3,3-diaminobenzidine (DAB). After DAB reaction, sections were rinsed two times in 0.1 M PBS, counterstained by Mayer’s hematoxylin, dehydrated using alcohols and xylene, and coverslipped with Entellan (Merck®).

For quantitative analysis of MBP and synaptophysin immunostaining, photomicrographs were acquired by an Axioscope microscope (Carl Zeiss, Germany) equipped with an AxioCam HRC CCD Color Camera (Carl Zeiss) with the same magnification of 40x. They were segmented by “deconvolution color plugin” (Gabriel Landini, http://www.dentistry.bham.ac.uk/landinig/software/software.html) using the ImageJ software (NIMH, NIH, Bethesda, MD, USA, https://imagej.nih.gov/ij/). Afterwards, the area fraction values (%) of DAB staining were measured in the sections [34, 37, 38]. The photomicrographs were acquired in the region of the second cerebellar leaf of 5 sections per animal, 3 micrographs per section (5 animals per group). The values obtained were expressed as .

2.5. Statistical Analyses

After data collection, all the results were tabulated and analyzed by GraphPad Prism 7.0 software (GraphPad Software Inc., La Jolla, CA, USA); the data distribution was tested by the Shapiro-Wilk method for verification of normality. The weight curve was evaluated with two-way ANOVA followed by the Tukey post hoc test. Oxidative biochemistry, Purkinje cell analysis, and behavioral assays were performed by one-way ANOVA and the Tukey post hoc test, except for immunohistochemical labeling, in which we use the nonparametric Mann-Whitney test, due to fraction area analysis. The results were expressed in of the mean (SEM), and values of were considered statistically significant.

3. Results

3.1. Repeated Cycles of EtOH in a Binge-Like Manner and Treadmill Physical Exercise Did Not Interfere in Animals’ Weight Gain

After repeated cycles of treadmill physical exercise and binge-like EtOH during 4 weeks, we did not observe alteration on animals’ weight (; Figure 3). At the end of the experiment, the animals had no difference in mean weight (control group: , exercised group: , EtOH group: , and exercised+EtOH group: ).

3.2. Regular Treadmill Physical Exercise Minimized Oxidative Stress Induced by Four Cycles of Binge Drinking in the Cerebellum of Rats

The binge-like EtOH exposure decreased TEAC levels (EtOH group: ; Figure 4(a)) in the cerebellum of rats compared to the control animals (; ). Such imbalance of oxidative biochemistry was avoided by physical exercise (exercised+EtOH group: 84.87; ; Figure 4(a)). In addition, as observed in Figure 4(b), exposure to EtOH did not change the oxidative parameters related to GSH levels () in the studied groups.

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Figure 4

Effects of repeated cycles of treadmill physical exercise and exposure to binge-like ethanol, during 4 weeks, on oxidative balance in the cerebellum of 60-day-old Wistar rats. (a) TEAC levels, (b) GSH levels, (c) percentages of milligram malondialdehyde per protein in relation to the control group, and (d) percentages of nitrite per milligram of protein in relation to the control group. Results are expressed as of mean. One-way ANOVA and Tukey’s post hoc test, . Statistical difference in relation to the other groups.

On the other hand, prooxidant factors were found to be statistically increased. The exposure to binge drinking of EtOH for 4 weeks was able to modulate oxidative biochemistry by increasing LPO levels (EtOH group: ) in the cerebellum of the animals, showing statistical difference compared to the control group (; ) as shown in Figure 4(c). In contrast, the physical exercise reduced the lipid peroxidation in the cerebellum, reflecting neuroprotection against EtOH disturbance (exercised+EtOH group: versus the control group; ). No difference in nitrite concentration among groups was detected (; Figure 4(d)).

3.3. Physical Exercise Reduced Cerebellar Tissue Damage Induced by Binge-Like EtOH Exposure for 4 Weeks in Rats

In order to analyze whether binge-like EtOH exposure can induce alterations in cerebellar tissue morphology, we performed HE staining. Our results show that binge-like EtOH exposure reduces Purkinje cell population in the rats (EtOH: ) when compared to the control group (control group: ; ; Figure 5). On the other hand, the physical exercise avoided the damage, once the trained groups did not show significant difference when compared to the control group (exercised group: ; exercised+EtOH group: ; ; Figure 5).

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Figure 5

Effects of repeated cycles of treadmill physical exercise and exposure to binge-like ethanol, during 4 weeks, on Purkinje cells in the cerebellum of 60-day-old Wistar rats. Representative photomicrographs of the (a) control group, (b) exercised group, (c) EtOH group, and (d) exercised+EtOH. Arrowheads indicate Purkinje cells. Results are expressed as of the number of cells counted per field (e). One-way ANOVA and Tukey’s post hoc test, . Statistical difference in relation to the other groups. Scale bar 20 μm.

In addition to the morphological changes caused by exposure to EtOH in a binge-like manner, we observed that the proposed pattern was able to decrease the area fraction of MBP immunostaining in the group exposed only to EtOH (EtOH group: ; ) compared to the other groups. Interestingly, the treadmill PE paradigm group reduced the loss of the MBP fraction area (exercised+EtOH group: ; control group: ; ), as seen in Figure 6, indicating a possible neuroprotection of the cerebellar damage of rats.

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Figure 6

Myelin basic protein (MBP) immunostaining in the cerebellum of 60-day-old Wistar rats exposed to repeated cycles of treadmill physical exercise and exposed to binge-like ethanol, during 4 weeks. Representative photomicrographs of the (a) control group, (b) exercised group, (c) EtOH group, and (d) exercised+EtOH. Results are expressed as of area fraction percentage of immunostaining (e). One-way ANOVA and Tukey’s post hoc test, . Statistical difference in relation to the other groups. Scale bar 20 μm.

In addition, we also demonstrated a remarkable decrease in the area fraction of synaptophysin immunostaining (), indicating that our model of exposure to EtOH causes damage to synaptic vesicles but can be minimized by treadmill physical exercise (control group: ; exercised+EtOH: ; ; Figure 7).

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Figure 7

Synaptophysin (SYP) immunostaining in the cerebellum of adult Wistar rats exposed to repeated cycles of treadmill physical exercise and exposure to binge-like ethanol during 4 weeks. Representative photomicrographs of the (a) control group, (b) exercised group, (c) EtOH group, and (d) exercised+EtOH. Results are expressed as of area fraction percentage of immunostaining (e). One-way ANOVA and Tukey’s post hoc test, . Statistical difference in relation to the other groups. Scale bar 20 μm.

3.4. Cerebellum Biochemical and Histological Alterations Reflected in Poor Performance on Spontaneous Locomotor Activity Assays of Rats after Binge-Like EtOH Exposure for 4 Weeks

Binge-like EtOH exposure for 4 weeks in rats induced spontaneous motor behavior deficits in behavioral tasks. However, the treadmill physical exercise triggered the performance of the spontaneous locomotor activity, both in the total distance travelled (control group: ; EtOH group: ; ; Figure 8(a)) and in the number of crossed quadrants (; EtOH group: ; ; Figure 8(b)) on the arena that was impaired by the EtOH protocol (), as observed in Figure 8.

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In the motor coordinating and balance test, subjects exposed to EtOH administration presented poor performance on the beam walking test, increasing the latency to reach the safe box, on both 12 mm and 5 mm beams (EtOH group: 12 mm: , ; control group: 12 mm: , ; 5 mm: , ; Figure 9(a)). In addition, the EtOH group increased the number of foot slips during the challenges with the smaller beam compared to other groups (5 mm: control group: ; exercised group: ; EtOH group: ; exercised+EtOH group: ; ; Figure 9(b)). However, the treadmill physical exercise improved the animal performance, reducing the latency to cross the beams, as well as the foot slips, restoring the accomplishment of the control individuals.

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4. Discussion

In this study, we demonstrated that EtOH exposure by means of 4 binge episodes from adolescence through adulthood is able to promote biochemical, tissue, and functional changes in the cerebellum of rats, which can be minimized due to the neuroprotection provided by concomitant treadmill physical exercise. The present findings demonstrate that the proposed binge ethanol drinking protocol induces oxidative biochemistry disbalance characterized by a decrease in TEAC levels and high lipid peroxidation, which can trigger tissue and behavioral damages in rats. In addition, we showed for the first time in literature that such damages in the cerebellum of adult rats promoted by binge drinking could be minimized by treadmill physical exercise.

Brain control of movements is performed by several regions of the CNS; among them, the cerebral cortex is responsible for motor planning and programming, in addition to command both from the spinal cord and brainstem that modulate reflexes and coarse movements [15]. A sensitive and accurate control system is performed by the cerebellum and basal ganglia [16, 39]. We have chosen the cerebellum as the investigation area since this structure modulates motor pathways and plans and coordinates movements, as well as motor learning [40].

In this context of motor control, the cerebellum is considered as the CNS region that integrates movement and posture, so as to control distance and range of motion [15]. In order to modulate movement, the afferent and efferent fibers of the cerebellar circuit communicate with the various regions of the nervous system (NS). The climbing and mossy fibers form excitatory synapses with deep cerebellar nuclei and Purkinje cells, directly or indirectly [16]. In our study, we evaluated the density of MBP, the synaptic communication pattern, and the preservation of Purkinje neurons in the cerebellar leaves.

Purkinje neurons were chosen to analyze the damage caused by ethanol, since they figure as the main elements of cerebellar circuitry; these cells are of fundamental importance in sensorimotor calibration due to their activity change during motor tasks in order to promote performance excellence [16]. In addition, Purkinje neurons can predict sensorimotor activities, transfer plasticity from cerebellar cortex to deep nuclei, and directly contribute to the motor command [40]. In our study, we found a decrease of Purkinje neuron density in animals exposed to ethanol, a fact that may have contributed to the functional damages observed.

Furthermore, we found an immunolabeling reduction of the synapse-related protein synaptophysin after 4 weeks of binge drinking; however, it is important to emphasize that cerebellar neurons are able to engage compensatory mechanisms to maintain neurotransmission at normal levels [15]. In addition, EtOH has been shown to promote alteration in other cell structures such as cytoplasmic organelles, nucleus, and cytoskeleton as well as changes in neuronal synaptic contacts in cell cultures of the cerebral cortex of rats [41].

Besides reducing Purkinje neuron density and synaptic communication, a binge EtOH pattern was able to damage the myelin sheath. It is known that damage to these structures can cause deficits in electrical impulse conduction during action potential in myelinated neurons, which are the main responsible for motor response [42]. We believe that these data gather evidence to support our hypothesis that binge-like ethanol exposure may trigger cerebellar damages, which can be suggested as responsible elements for motor impairment found in our investigation.

Deficiency in thiamine synthesis, retinoic acid modulation, and neuroinflammation can be identified as responsible elements for CNS damages caused by EtOH, whereas oxidative stress is a strong mechanism also associated with damage as described by the previous studies [5, 43, 44]. Oxidative stress results from an imbalance between production and removal of reactive oxygen species and causes severe cellular damage that may lead—after following hierarchical damages—to behavioral alterations [44].

Although the literature indicates oxidative stress as one of the main mechanisms of CNS damage caused by EtOH [45–47], our research is the first to describe the oxidative changes in the cerebellum of rats triggered by the binge pattern and EtOH consumption from adolescence through adulthood in rats. We have shown that even with 4 binge episodes during this period, there is a decrease in total antioxidant capacity (TEAC) and an increase in lipid peroxidation (MDA) levels on the cerebellum; this was previously reported by other studies but through chronic consumption [44, 45, 48]. Even though neural damage mechanisms have not yet been fully understood, oxidative stress is considered one of the most relevant [46, 49]. EtOH intoxication due to acute, chronic, or binge exposure leads to oxidative stress as a result of an imbalance between free radicals (FRs) and antioxidants, followed by the formation of reactive species (RSs) and lipid peroxidation [43, 44]. Recently, our group also showed that ethanol exposure led to oxidative stress with increased MDA levels, possibly reflecting on the CNS, which led to a profile of psychiatric disorders or cognitive impairment [50]. Thus, this increase in the levels of MDA caused by ethanol consumption may be associated with a higher CAT activity induced by the ethanol metabolic process, which generates excess H₂O₂ and hydroxyl radicals (OH^•), one of the most potent molecules leading to oxidation and damage to biomolecules [51]. Therefore, we confirm here that the toxicity of ethanol during its metabolism leads to a marked oxidative stress due to the powerful oxidizing molecules in the body.

When we evaluated the motor activity of our exposure model (binge), the results indicated that EtOH promoted changes in exploratory capacity, since the exposed animals walked through smaller number of quadrants and shorter total distance. Moreover, at the beam walking test, animals that consumed alcohol presented worse motor performance characterized by higher number of falls and less ability for activities that required motor dexterity. These results corroborate those recently found in another study from our group, which demonstrated that binge episodes during adolescence impact motor performance [50, 52], as well as affect other brain areas, inducing an anxiogenic behavior and influencing short-term recognition memory [6]. We believe that ethanol consumption increased cerebral blood flow by increasing the metabolic oxygen demand, so that brain regions related to motor activity and central command network are affected. Thus, increased oxygen consumption results in increased production of a variety of reactive oxygen species (ROS), such as superoxide anion, OH^•, nitric oxide (NO), and singlet oxygen due to oxidation leakage of electrons related to the mitochondrial transport chain phosphorylation [50, 53]. Thus, ROS can cause oxidative damage to cellular components by impairing cellular energy and modulating signaling pathways (“redox signaling”) that lead to several acute and chronic changes in the cellular environment, especially in the CNS [54]. Thereby, overall, the decreased antioxidant levels and increase of ROS in the cerebellum may be responsible for cellular damage, including in Purkinje neurons, which resulted in impairment in motor activity, anxiogenic behavior, and a short-term recognition memory failure.

On the other hand, there are some strategies to reverse, minimize, or protect the CNS from alterations caused by oxidative stress [55–57]. Among neuroprotection strategies, exercise is a subcategory of physical activity that involves several exercise methods such as volume, frequency, and intensity. Moreover, muscular strengthening exercise mostly relies on anaerobic energy sources [58]. On the contrary, exercises of aerobic resistance generally use mainly aerobic energy sources, so they were chosen for this research [56]. Thus, regular moderate exercise is an essential factor for maintaining good health and helps in the prevention of chronic diseases, including neurodegeneration [59–61]. In this regard, several studies reported that moderate regular exercise beneficially stimulates brain function by playing an important preventive and therapeutic role in cases of stroke and in Alzheimer’s and Parkinson’s diseases [62–65]. However, these beneficial effects are broad, including the stimulation of neurogenesis via neurotrophic factors, increased capillarization, decreased oxidative damage, and increased proteolytic degradation by proteasome and neprilysin, among others [66].

Although muscular strengthening exercises present some relevance regarding the improvement of CNS-related parameters such as motor skills, balance, and coordination [67], we have chosen aerobic exercise as a possible oxidative stress modulator in the cerebellum due to its ability to modulate oxidative stress in other tissues, such as blood [68, 69]. In our investigation, moderate treadmill physical exercise was able to modulate enzymatic and nonenzymatic antioxidant parameters in the cerebellum, which has led to the maintenance of T-AOC and avoided oxidative stress by increasing lipid peroxidation. A study on the effect of low-intensity aerobic exercise in humans after 12 and 24 months reported that aerobic exercise was able to modulate antioxidant enzymes; however, this seems to be time dependent since only a difference in superoxide dismutase (SOD) activity was observed at 12 months, while differences in catalase activity (CAT) and reduction of blood lipid peroxidation were found after 24 months of exercise [69]. However, the effect of exercise on activities of antioxidant enzymes is still conflicting. Some authors report that the effects of exercise on antioxidant enzyme activities are dependent on the brain region, in certain parts such as the stem and corpus striatum; physical exercise resulted in increased activities of superoxide dismutase (SOD) and GPX [70, 71], while other brain areas do not change, as in the cerebellum. Another important aspect is that PE by treadmill running also did not alter the activities of SOD, catalase, or GPX in the rat brain [66]. In general, it is suggested that physical exercise selectively regulates the antioxidant factors, mainly enzymatic, in different regions of the brain, which is probably dependent on the type of physical activity, intensity and duration of physical exercise, age, sex, and strain of rats [66].

In an animal study, a 6-week aerobic training protocol of moderate intensity did not result in SOD difference; however, CAT alterations and oxidative stress reduction in regions associated with motor functions such as motor cortex, cerebellum, and striatum were observed [68]. No improvement was noted by using our protocol comprised of 4 weeks of aerobic resistance exercise with moderate intensity [18]; however, the antioxidant parameters were not altered in comparison to the control group, which indicates a protective effect on the antioxidant activity [68]. Exercise was not able to improve the antioxidant system capacity of animals that did not consume EtOH; this can be explained by the fact that antioxidant responses depend on the exercise type, volume, and intensity of the training protocol [72].

Although the antioxidant parameters were not improved, the exercise protocol was able to avoid the reduction of myelin basic protein (MBP) immunolabeling, as well as the decrease of Purkinje cell density caused by the EtOH consumption. These results may arise from the ability of moderate aerobic exercise to increase the expression of neurotrophic factors such as BDNF (brain-derived neurotrophic factor), FGF (fibroblast growth factor), and VEGF (vascular endothelial growth factor), which are responsible for development and maintenance of the nervous system [6, 73]. The physical exercise has modulatory effects on energy hemostasis through improving mitochondrial function, leading to increased levels of BDNF that activates the transcription factor cAMP response element-binding protein (CREB) through tyrosine-related kinase B (TrkB) receptors. This process creates a positive cycle for the cascades, as well as induces autophagy and promotes the antioxidant system, making the neurons more resistant to oxidative stress by activating regulatory mechanisms in that cell [74]. DNA-binding sites for CREB contribute the increase in the expression of BDNF mRNA, a process that can be regulated by ROS; thus, the increase of ROS stimulates the expression of these factors that play a key role in the control of the oxidative process [66, 75]. Therefore, exercise can lead to redox homeostasis by stimulating the regulatory process.

In this regard, regular physical exercise attenuated the accumulation of carbonylated proteins related to oxidative stress in the brain associated with age, due to increased proteasome activity and thus improved brain function [76]. Studies with transgenic mice that accumulate beta-amyloid proteins have also shown that voluntary exercise decreased beta-amyloid accumulation in the brain by increasing the activity of neprilysin, a protein responsible for the degradation of beta-amyloids [77]. Thereby, biochemical changes can lead to cellular damage and result in motor activity reduction, as seen in this study. In contrast, physical exercise was able to protect the CNS from all changes caused by EtOH, including biochemical, cellular, and behavioral damages. This entire protection is conditioned to the exercise capacity to modulate the antioxidant process, growth factors, and maintenance of CNS [6, 73].

5. Conclusions

We demonstrated that that EtOH in binge drinking induces oxidative biochemistry imbalance, from the decrease of TEAC levels and higher LPO production, which can trigger tissue and behavioral damages in rats. Finally, the decrease in the number of cells was probably the main factor responsible for changes on fine motor control—the main findings of this paper are summarized in Figure 10. One of the main contributions of the present study was to show for the first time that the damages promoted in the cerebellum of rats by EtOH binge drinking can be minimized by moderate treadmill physical exercise, avoiding the oxidative imbalance and minimizing the tissue and functional damages in the cerebellum caused by ethanol, possibly leading to redox homeostasis by stimulating the regulatory process.

Figure 10

Description of the main results found in this article. Exposure to binge-like ethanol caused lower antisynaptophysin (SYP) immunostaining and oxidative stress, from the decrease of Trolox equivalent antioxidant capacity (TEAC) levels and higher lipid peroxidation (LPO). The oxidative biochemistry misbalance induced tissue damages, as decrease of Purkinje cell population and antimyelin basic protein (MBP) immunostaining. The oxidative biochemistry and tissue damage were the main factors responsible for fine motor control changes. In addition, we indicated the role of physical exercise in damage ways in exposure to binge-like ethanol.

Data Availability

The quantitative and qualitative data used to support the findings of this study are included within the article.

Conflicts of Interest

Rafael R. Lima is an investigator from CNPq (Edital MCTI/CNPQ/Universal 14/2014). The authors declare no conflict of interest.

Authors’ Contributions

Kátia Lamarão-Vieira and Dinair Pamplona-Santos contributed equally to this work.

Acknowledgments

This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq) and Pró-Reitoria de Pesquisa e Pós-Graduação da UFPA (PROPESP, UFPA, Brazil). This paper had financial support from the CNPq—Brazilian Ministry of Science, Technology and Innovation, through MCTI/CNPq/Universal 14/2014.

References

World Health Organization, Global Status Report on Alcohol and Health, World Health Organization, 2014.
J. Rehm and S. Imtiaz, “A narrative review of alcohol consumption as a risk factor for global burden of disease,” Substance Abuse Treatment, Prevention, and Policy, vol. 11, no. 1, p. 37, 2016.
View at: Publisher Site | Google Scholar
A. Klintsova, G. Hamilton, and K. Boschen, “Long-term consequences of developmental alcohol exposure on brain structure and function: therapeutic benefits of physical activity,” Brain Sciences, vol. 3, no. 4, pp. 1–38, 2013.
View at: Publisher Site | Google Scholar
M. A. Schuckit, “Alcohol-use disorders,” The Lancet, vol. 373, no. 9662, pp. 492–501, 2009.
View at: Publisher Site | Google Scholar
F. B. Teixeira, L. N. da Silva Santana, F. R. Bezerra et al., “Chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress,” PLoS One, vol. 9, no. 6, article e101074, 2014.
View at: Publisher Site | Google Scholar
L. M. P. Fernandes, S. C. Cartágenes, M. A. Barros et al., “Repeated cycles of binge-like ethanol exposure induce immediate and delayed neurobehavioral changes and hippocampal dysfunction in adolescent female rats,” Behavioural Brain Research, vol. 350, pp. 99–108, 2018.
View at: Publisher Site | Google Scholar
M. Pascual, J. Montesinos, M. Marcos et al., “Gender differences in the inflammatory cytokine and chemokine profiles induced by binge ethanol drinking in adolescence,” Addiction Biology, vol. 22, no. 6, pp. 1829–1841, 2017.
View at: Publisher Site | Google Scholar
Y. H. Ding, Y. Ding, J. Li, D. A. Bessert, and J. A. Rafols, “Exercise pre-conditioning strengthens brain microvascular integrity in a rat stroke model,” Neurological Research, vol. 28, no. 2, pp. 184–189, 2006.
View at: Publisher Site | Google Scholar
C. Pin-Barre and J. Laurin, “Physical exercise as a diagnostic, rehabilitation, and preventive tool: influence on neuroplasticity and motor recovery after stroke,” Neural Plasticity, vol. 2015, Article ID 608581, 12 pages, 2015.
View at: Publisher Site | Google Scholar
R. M. Fernandes, M. G. Correa, M. A. R. dos Santos et al., “The effects of moderate physical exercise on adult cognition: a systematic review,” Frontiers in Physiology, vol. 9, p. 667, 2018.
View at: Publisher Site | Google Scholar
S. Perrey, “Promoting motor function by exercising the brain,” Brain Sciences, vol. 3, no. 4, pp. 101–122, 2013.
View at: Publisher Site | Google Scholar
M. Subramanian, A. G. Holt, and P. J. Mueller, “Physical activity correlates with glutamate receptor gene expression in spinally-projecting RVLM neurons: a laser capture microdissection study,” Brain Research, vol. 1585, pp. 51–62, 2014.
View at: Publisher Site | Google Scholar
R. J. Maddock, G. A. Casazza, D. H. Fernandez, and M. I. Maddock, “Acute modulation of cortical glutamate and GABA content by physical activity,” The Journal of Neuroscience, vol. 36, no. 8, pp. 2449–2457, 2016.
View at: Publisher Site | Google Scholar
F. B. R. da Silva, P. A. Cunha, P. C. Ribera et al., “Heavy chronic ethanol exposure from adolescence to adulthood induces cerebellar neuronal loss and motor function damage in female rats,” Frontiers in Behavioral Neuroscience, vol. 12, p. 88, 2018.
View at: Publisher Site | Google Scholar
C. F. Valenzuela and K. Jotty, “Mini-review: effects of ethanol on GABAA receptor-mediated neurotransmission in the cerebellar cortex--recent advances,” Cerebellum, vol. 14, no. 4, pp. 438–446, 2015.
View at: Publisher Site | Google Scholar
C. F. Valenzuela, B. Lindquist, and P. A. Zamudio-Bulcock, “A review of synaptic plasticity at Purkinje neurons with a focus on ethanol-induced cerebellar dysfunction,” International Review of Neurobiology, vol. 91, pp. 339–372, 2010.
View at: Publisher Site | Google Scholar
National Research Council, Guide for the Care and Use of Laboratory Animals, The National Academies Press, Washington, DC, 8th edition, 2011.
R. M. Arida, F. A. Scorza, A. F. S. de Lacerda, S. G. da Silva, and E. A. Cavalheiro, “Physical training in developing rats does not influence the kindling development in the adult life,” Physiology & Behavior, vol. 90, no. 4, pp. 629–633, 2007.
View at: Publisher Site | Google Scholar
K. Lauing, R. Himes, M. Rachwalski, P. Strotman, and J. J. Callaci, “Binge alcohol treatment of adolescent rats followed by alcohol abstinence is associated with site-specific differences in bone loss and incomplete recovery of bone mass and strength,” Alcohol, vol. 42, no. 8, pp. 649–656, 2008.
View at: Publisher Site | Google Scholar
C. Lindtner, T. Scherer, E. Zielinski et al., “Binge drinking induces whole-body insulin resistance by impairing hypothalamic insulin action,” Science Translational Medicine, vol. 5, no. 170, article 170ra14, 2013.
View at: Publisher Site | Google Scholar
R. N. Walsh and R. A. Cummins, “The open-field test: a critical review,” Psychological Bulletin, vol. 83, no. 3, pp. 482–504, 1976.
View at: Publisher Site | Google Scholar
F. B. Teixeira, A. C. A. de Oliveira, L. K. R. Leão et al., “Exposure to inorganic mercury causes oxidative stress, cell death, and functional deficits in the motor cortex,” Frontiers in Molecular Neuroscience, vol. 11, no. 125, 2018.
View at: Publisher Site | Google Scholar
T. Karl, R. Pabst, and S. von Horsten, “Behavioral phenotyping of mice in pharmacological and toxicological research,” Experimental and Toxicologic Pathology, vol. 55, no. 1, pp. 69–83, 2003.
View at: Publisher Site | Google Scholar
A. C. A. Oliveira, M. C. S. Pereira, L. N. da Silva Santana et al., “Chronic ethanol exposure during adolescence through early adulthood in female rats induces emotional and memory deficits associated with morphological and molecular alterations in hippocampus,” Journal of Psychopharmacology, vol. 29, no. 6, pp. 712–724, 2015.
View at: Publisher Site | Google Scholar
M. S. M. Rufino, R. E. Alves, E. S. Brito et al., Metodologia Científica: Determinação da Atividade Antioxidante Total em Frutas pela Captura do Radical Livre ABTS°+, Embrapa Agroindústria Tropical, Fortaleza, 2007.
G. H. Miranda, B. Gomes, L. Bittencourt et al., Chronic exposure to sodium fluoride triggers oxidative biochemistry misbalance in mice, effects in blood peripheral circulation, 2018.
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Radical Biology & Medicine, vol. 26, no. 9-10, pp. 1231–1237, 1999.
View at: Publisher Site | Google Scholar
G. L. Ellman, “Tissue sulfhydryl groups,” Archives of Biochemistry and Biophysics, vol. 82, no. 1, pp. 70–77, 1959.
View at: Publisher Site | Google Scholar
T. R. Schalcher, R. S. Borges, M. D. Coleman et al., “Clinical oxidative stress during leprosy multidrug therapy: impact of dapsone oxidation,” PLoS One, vol. 9, no. 1, article e85712, 2014.
View at: Publisher Site | Google Scholar
H. Esterbauer and K. H. Cheeseman, “[42] Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal,” Methods in Enzymology, vol. 186, pp. 407–421, 1990.
View at: Publisher Site | Google Scholar
L. O. Bittencourt, B. Puty, S. Charone et al., “Oxidative biochemistry disbalance and changes on proteomic profile in salivary glands of rats induced by chronic exposure to methylmercury,” Oxidative Medicine and Cellular Longevity, vol. 2017, Article ID 5653291, 15 pages, 2017.
View at: Publisher Site | Google Scholar
L. C. Green, S. R. Tannenbaum, and P. Goldman, “Nitrate synthesis in the germfree and conventional rat,” Science, vol. 212, no. 4490, pp. 56–58, 1981.
View at: Google Scholar
M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976.
View at: Publisher Site | Google Scholar
R. R. Lima, L. N. S. Santana, R. M. Fernandes et al., “Neurodegeneration and glial response after acute striatal stroke: histological basis for neuroprotective studies,” Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 3173564, 15 pages, 2016.
View at: Publisher Site | Google Scholar
W. A. B. Aragao, F. B. Teixeira, N. C. F. Fagundes et al., “Hippocampal dysfunction provoked by mercury chloride exposure: evaluation of cognitive impairment, oxidative stress, tissue injury and nature of cell death,” Oxidative Medicine and Cellular Longevity, vol. 2018, Article ID 7878050, 11 pages, 2018.
View at: Publisher Site | Google Scholar
L. N. d. S. Santana, L. O. Bittencourt, P. C. Nascimento et al., “Low doses of methylmercury exposure during adulthood in rats display oxidative stress, neurodegeneration in the motor cortex and lead to impairment of motor skills,” Journal of Trace Elements in Medicine and Biology, vol. 51, pp. 19–27, 2019.
View at: Publisher Site | Google Scholar
N. C. F. Fagundes, L. M. P. Fernandes, R. S. de Oliveira Paraense et al., “Binge drinking of ethanol during adolescence induces oxidative damage and morphological changes in salivary glands of female rats,” Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 7323627, 11 pages, 2016.
View at: Publisher Site | Google Scholar
L. M. Fernandes, F. B. Teixeira, S. M. Alves-Junior, J. Pinheiro Jde, C. S. Maia, and R. R. Lima, “Immunohistochemical changes and atrophy after chronic ethanol intoxication in rat salivary glands,” Histology and Histopathology, vol. 30, no. 9, pp. 1069–1078, 2015.
View at: Publisher Site | Google Scholar
T. Paillard, Y. Rolland, and P. de Souto Barreto, “Protective effects of physical exercise in Alzheimer’s disease and Parkinson’s disease: a narrative review,” Journal of Clinical Neurology, vol. 11, no. 3, pp. 212–219, 2015.
View at: Publisher Site | Google Scholar
J. F. Medina, “The multiple roles of Purkinje cells in sensori-motor calibration: to predict, teach and command,” Current Opinion in Neurobiology, vol. 21, no. 4, pp. 616–622, 2011.
View at: Publisher Site | Google Scholar
T. Guadagnoli, L. Caltana, M. Vacotto, M. M. Gironacci, and A. Brusco, “Direct effects of ethanol on neuronal differentiation: an in vitro analysis of viability and morphology,” Brain Research Bulletin, vol. 127, pp. 177–186, 2016.
View at: Publisher Site | Google Scholar
E. D. Buttermore, C. L. Thaxton, and M. A. Bhat, “Organization and maintenance of molecular domains in myelinated axons,” Journal of Neuroscience Research, vol. 91, no. 5, pp. 603–622, 2013.
View at: Publisher Site | Google Scholar
F. Yang and J. Luo, “Endoplasmic reticulum stress and ethanol neurotoxicity,” Biomolecules, vol. 5, no. 4, pp. 2538–2553, 2015.
View at: Publisher Site | Google Scholar
T. E. Muller, S. Z. Nunes, A. Silveira, V. L. Loro, and D. B. Rosemberg, “Repeated ethanol exposure alters social behavior and oxidative stress parameters of zebrafish,” Progress in Neuro-Psychopharmacology and Biological Psychiatry, vol. 79, no. Part B, pp. 105–111, 2017.
View at: Publisher Site | Google Scholar
J. F. Agostini, H. C. Z. D. Toé, K. M. Vieira et al., “Cholinergic system and oxidative stress changes in the brain of a zebrafish model chronically exposed to ethanol,” Neurotoxicity Research, vol. 33, no. 4, pp. 749–758, 2018.
View at: Publisher Site | Google Scholar
C. Tapia-Rojas, R. G. Mira, A. K. Torres et al., “Alcohol consumption during adolescence: a link between mitochondrial damage and ethanol brain intoxication,” Birth Defects Research, vol. 109, no. 20, pp. 1623–1639, 2017.
View at: Publisher Site | Google Scholar
J. Luo, “Autophagy and ethanol neurotoxicity,” Autophagy, vol. 10, no. 12, pp. 2099–2108, 2015.
View at: Publisher Site | Google Scholar
A. Hamid, F. W. Ibrahim, T. H. Ming et al., “Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity,” BMC Complementary and Alternative Medicine, vol. 18, no. 1, p. 101, 2018.
View at: Publisher Site | Google Scholar
J. A. Hernandez, R. C. Lopez-Sanchez, and A. Rendon-Ramirez, “Lipids and oxidative stress associated with ethanol-induced neurological damage,” Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 1543809, 15 pages, 2016.
View at: Publisher Site | Google Scholar
I. J. A. Belem-Filho, P. C. Ribera, A. L. Nascimento et al., “Low doses of methylmercury intoxication solely or associated to ethanol binge drinking induce psychiatric-like disorders in adolescent female rats,” Environmental Toxicology and Pharmacology, vol. 60, pp. 184–194, 2018.
View at: Publisher Site | Google Scholar
B. Halliwell, “Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life,” Plant Physiology, vol. 141, no. 2, pp. 312–322, 2006.
View at: Publisher Site | Google Scholar
A. N. Oliveira, A. M. Pinheiro, I. J. A. Belem-Filho et al., “Unravelling motor behaviour hallmarks in intoxicated adolescents: methylmercury subtoxic-dose exposure and binge ethanol intake paradigm in rats,” Environmental Science and Pollution Research International, vol. 25, no. 22, pp. 21937–21948, 2018.
View at: Publisher Site | Google Scholar
A. P. Kudin, D. Malinska, and W. S. Kunz, “Sites of generation of reactive oxygen species in homogenates of brain tissue determined with the use of respiratory substrates and inhibitors,” Biochimica et Biophysica Acta (BBA) - Bioenergetics, vol. 1777, no. 7-8, pp. 689–695, 2008.
View at: Publisher Site | Google Scholar
M. Shichiri, “The role of lipid peroxidation in neurological disorders,” Journal of Clinical Biochemistry and Nutrition, vol. 54, no. 3, pp. 151–160, 2014.
View at: Publisher Site | Google Scholar
D. B. Rosemberg, R. F. da Rocha, E. P. Rico et al., “Taurine prevents enhancement of acetylcholinesterase activity induced by acute ethanol exposure and decreases the level of markers of oxidative stress in zebrafish brain,” Neuroscience, vol. 171, no. 3, pp. 683–692, 2010.
View at: Publisher Site | Google Scholar
J. H. Han, H. Z. Tian, Y. Y. Lian et al., “Quetiapine mitigates the ethanol-induced oxidative stress in brain tissue, but not in the liver, of the rat,” Neuropsychiatric Disease and Treatment, vol. 11, pp. 1473–1482, 2015.
View at: Publisher Site | Google Scholar
W. Luczaj, I. Jarocka-Karpowicz, K. Bielawska, and E. Skrzydlewska, “Sweet grass protection against oxidative stress formation in the rat brain,” Metabolic Brain Disease, vol. 30, no. 1, pp. 183–190, 2015.
View at: Publisher Site | Google Scholar
F. Cechetti, P. V. Worm, V. R. Elsner et al., “Forced treadmill exercise prevents oxidative stress and memory deficits following chronic cerebral hypoperfusion in the rat,” Neurobiology of Learning and Memory, vol. 97, no. 1, pp. 90–96, 2012.
View at: Publisher Site | Google Scholar
J. A. Zoladz, J. Majerczak, E. Zeligowska et al., “Moderate-intensity interval training increases serum brain-derived neurotrophic factor level and decreases inflammation in Parkinson’s disease patients,” Journal of Physiology and Pharmacology, vol. 65, no. 3, pp. 441–448, 2014.
View at: Google Scholar
R. J. Dougherty, S. A. Schultz, T. K. Kirby et al., “Moderate physical activity is associated with cerebral glucose metabolism in adults at risk for Alzheimer’s disease,” Journal of Alzheimer's Disease, vol. 58, no. 4, pp. 1089–1097, 2017.
View at: Publisher Site | Google Scholar
A. Marques, M. Peralta, J. Martins, E. R. Gouveia, and M. G. Valeiro, “Cross-sectional and prospective relationship between low-to-moderate-intensity physical activity and chronic diseases in older adults from 13 European countries,” Journal of Aging and Physical Activity, pp. 1–9, 2018.
View at: Publisher Site | Google Scholar
R. Molteni, J. Q. Zheng, Z. Ying, F. Gomez-Pinilla, and J. L. Twiss, “Voluntary exercise increases axonal regeneration from sensory neurons,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 22, pp. 8473–8478, 2004.
View at: Publisher Site | Google Scholar
S. A. Neeper, F. Góauctemez-Pinilla, J. Choi, and C. Cotman, “Exercise and brain neurotrophins,” Nature, vol. 373, no. 6510, p. 109, 1995.
View at: Publisher Site | Google Scholar
H. Ogonovszky, I. Berkes, S. Kumagai et al., “The effects of moderate-, strenuous- and over-training on oxidative stress markers, DNA repair, and memory, in rat brain,” Neurochemistry International, vol. 46, no. 8, pp. 635–640, 2005.
View at: Publisher Site | Google Scholar
H. Ogonovszky, M. Sasvari, A. Dosek et al., “The effects of moderate, strenuous, and overtraining on oxidative stress markers and DNA repair in rat liver,” Canadian Journal of Applied Physiology, vol. 30, no. 2, pp. 186–195, 2005.
View at: Publisher Site | Google Scholar
Z. Radak, S. Kumagai, A. W. Taylor, H. Naito, and S. Goto, “Effects of exercise on brain function: role of free radicals,” Applied Physiology, Nutrition, and Metabolism, vol. 32, no. 5, pp. 942–946, 2007.
View at: Publisher Site | Google Scholar
C. E. Garber, B. Blissmer, M. R. Deschenes et al., “American College of Sports Medicine position stand. Quantity and quality of exercise for developing and maintaining cardiorespiratory, musculoskeletal, and neuromotor fitness in apparently healthy adults: guidance for prescribing exercise,” Medicine and Science in Sports and Exercise, vol. 43, no. 7, pp. 1334–1359, 2011.
View at: Publisher Site | Google Scholar
M. Chalimoniuk, S. Jagsz, E. Sadowska-Krepa, S. J. Chrapusta, B. Klapcinska, and J. Langfort, “Diversity of endurance training effects on antioxidant defenses and oxidative damage in different brain regions of adolescent male rats,” Journal of Physiology and Pharmacology, vol. 66, no. 4, pp. 539–547, 2015.
View at: Google Scholar
B. Hajizadeh Maleki, B. Tartibian, F. C. Mooren et al., “Low-to-moderate intensity aerobic exercise training modulates irritable bowel syndrome through antioxidative and inflammatory mechanisms in women: results of a randomized controlled trial,” Cytokine, vol. 102, pp. 18–25, 2018.
View at: Publisher Site | Google Scholar
L. P. Rybak, S. M. Somani, and R. Ravi, “Effect of exercise training on antioxidant system in brain regions of rat,” Pharmacology, Biochemistry, and Behavior, vol. 50, no. 4, pp. 635–639, 1995.
View at: Publisher Site | Google Scholar
Z. Radak, A. Toldy, Z. Szabo et al., “The effects of training and detraining on memory, neurotrophins and oxidative stress markers in rat brain,” Neurochemistry International, vol. 49, no. 4, pp. 387–392, 2006.
View at: Publisher Site | Google Scholar
D. Camiletti-Moiron, V. A. Aparicio, P. Aranda, and Z. Radak, “Does exercise reduce brain oxidative stress? A systematic review,” Scandinavian Journal of Medicine & Science in Sports, vol. 23, no. 4, pp. e202–e212, 2013.
View at: Publisher Site | Google Scholar
D. Saraulli, M. Costanzi, V. Mastrorilli, and S. Farioli-Vecchioli, “The long run: neuroprotective effects of physical exercise on adult neurogenesis from youth to old age,” Current Neuropharmacology, vol. 15, no. 4, pp. 519–533, 2017.
View at: Publisher Site | Google Scholar
H. Bo, N. Jiang, L. L. Ji, and Y. Zhang, “Mitochondrial redox metabolism in aging: effect of exercise interventions,” Journal of Sport and Health Science, vol. 2, no. 2, pp. 67–74, 2013.
View at: Publisher Site | Google Scholar
M. Peeri and S. Amiri, “Protective effects of exercise in metabolic disorders are mediated by inhibition of mitochondrial-derived sterile inflammation,” Medical Hypotheses, vol. 85, no. 6, pp. 707–709, 2015.
View at: Publisher Site | Google Scholar
Z. Radak, T. Kaneko, S. Tahara et al., “Regular exercise improves cognitive function and decreases oxidative damage in rat brain,” Neurochemistry International, vol. 38, no. 1, pp. 17–23, 2001.
View at: Publisher Site | Google Scholar
O. Lazarov, J. Robinson, Y.-P. Tang et al., “Environmental enrichment reduces Abeta levels and amyloid deposition in transgenic mice,” Cell, vol. 120, no. 5, pp. 701–713, 2005.
View at: Publisher Site | Google Scholar

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