Research Article

Tetramethylpyrazine Prevents Contrast-Induced Nephropathy via Modulating Tubular Cell Mitophagy and Suppressing Mitochondrial Fragmentation, CCL2/CCR2-Mediated Inflammation, and Intestinal Injury

Figure 3

Analysis of LC3B, beclin-1, and p62 protein abundance and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The autophagy-associated proteins LC3B and beclin-1 were detected by western blotting (a). The abundance of LC3B-II (b), beclin-1 (c), and p62 (d) was quantified by densitometry and normalized to that of LC3B-I or GAPDH, respectively. Renal tubular cell apoptosis was assessed by TUNEL (e) staining in Con (E1), CIN (E2), CIN+NAC (E3), and CIN+TMP (E4) rats. Semiquantitative analysis of TUNEL staining (f). Figures are representative of 5–8 rats from each group. Data are represented as (). versus Con and ## versus CIN.
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