Synthetic Imine Resveratrol Analog 2-Methoxyl-3,6-Dihydroxyl-IRA Ameliorates Colitis by Activating Protective Nrf2 Pathway and Inhibiting NLRP3 Expression

Figure 2

C33 ameliorates DSS-induced colitis in WT but not Nrf2^-/- mice. (a) Experimental protocol for C33 and RSV treatment in DSS-induced colitis. WT and Nrf2^-/- mice were given C33 (200 mg/kg i.g.), RSV (200 mg/kg i.g.), or vehicle (10% ethanol) daily. Two days later, while the mice continued daily treatment with RSV or C33, they were also given normal drinking water or water containing 2.5% DSS for 7 days. (b) Evaluation of the body weight of WT and Nrf2^-/- mice after treatment as in (a). Initial body weight was set as 1. (c) Colon length from mice treated as in (a). Colon length is represented as a ratio (cm/g) relative to the starting weight of mice prior to DSS administration (). (d) Representative H&E staining of colon sections from mice treated as in (a) (scale bar, 50 μm; original magnification, ×40). WT, A–D; Nrf2^-/-, E–H. Water (A and E), mice on normal drinking water without any treatment. +DSS (B–D and F–H), mice on drinking water containing 2.5% DSS. (e) Combined scores for the severity of crypt damage (, ; , ). (f) IHC staining of colonic sections with anti-nitrogen tyrosine (scale bar, 100 μm; original magnification, ×200). WT, A–D; Nrf2^-/-, E–H. Water (A and E), mice on normal drinking water without any treatment. +DSS (B–D and F–H), mice on drinking water containing 2.5% DSS. The WT mice on normal drinking water (control) was set at 1. Values are (; , ).

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