Shepherd’s Purse Polyphenols Exert Its Anti-Inflammatory and Antioxidative Effects Associated with Suppressing MAPK and NF-κB Pathways and Heme Oxygenase-1 Activation

Figure 5

SPE inhibited the activation of LPS-induced MAPK (JNK, ERK, and p38) and NF-κB (IκB α and p65). Cells were incubated with 1 μg/ml LPS with the addition of different concentration of SPE for 1 h; the protein were extracted and the phosphorylation of JNK, ERK, p 38, p 65, and IκB α were analyzed with western blot assay. (a, c) Western blot assay of activation of MAPKs and NF-κB were analyzed by and (b, d) The band intensity of phosphorylation MAPKs and NF-κB were normalized with nonphosphorylation of MAPKs and β-actin. and versus LPS alone group.

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