MLN4924 Exerts a Neuroprotective Effect against Oxidative Stress via Sirt1 in Spinal Cord Ischemia-Reperfusion Injury

<div>MLN4924 protects against H<sub>2</sub>O<sub>2</sub>-induced apoptosis of SH-SY5Y cells via Sirt1. (a) TUNEL immunostaining in SH-SY5Y cells treated with or without H<sub>2</sub>O<sub>2</sub> and MLN4924/EX527. (b–g) Western blot analysis of Sirt1, acetylated FoxO1, BCL2, Bax, and cleaved caspase-3 in SH-SY5Y cells treated with or without H<sub>2</sub>O<sub>2</sub> and MLN4924/Sirt1-siRNA. 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166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,73.667,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>.</span> <svg height="8.68572pt" id="M32" style="vertical-align:-0.0498209pt" version="1.1" viewbox="-0.0498162 -8.6359 8.15071 8.68572" width="8.15071pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g></svg> values were analyzed by one-way ANOVA. Scale bar represents 50 <i>μ</i>m.</div>

Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: MLN4924 Exerts a Neuroprotective Effect against Oxidative Stress via Sirt1 in Spinal Cord Ischemia-Reperfusion Injury 