MLN4924 Exerts a Neuroprotective Effect against Oxidative Stress via Sirt1 in Spinal Cord Ischemia-Reperfusion Injury
MLN4924 protects against H2O2-induced apoptosis of SH-SY5Y cells via Sirt1. (a) TUNEL immunostaining in SH-SY5Y cells treated with or without H2O2 and MLN4924/EX527. (b–g) Western blot analysis of Sirt1, acetylated FoxO1, BCL2, Bax, and cleaved caspase-3 in SH-SY5Y cells treated with or without H2O2 and MLN4924/Sirt1-siRNA. H2O2: 200 μM; MLN4924: 110 nM; Ex527: 1 μM. ,,, and . values were analyzed by one-way ANOVA. Scale bar represents 50 μm.
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