Research Article

Resveratrol Attenuates Oxidative Stress-Induced Intestinal Barrier Injury through PI3K/Akt-Mediated Nrf2 Signaling Pathway

Figure 3

Effects of resveratrol and/or H2O2 on the levels of TJ proteins. IPEC-J2 cells were pretreated with the indicated concentrations of RSV and then cocultured with 500 μM H2O2 for 4 hours. (a) The expression of claudin-1, occludin, and ZO-1 was detected by quantitative real-time PCR (qRT-PCR). Data are shown as ratios of abundance of target gene transcripts in the treated cells to those in the control cells after normalization to β-actin. (b) Protein levels of claudin-1, occludin, and ZO-1 were detected by Western blot with β-actin as the loading control. Values are the ; . The columns with the same superscript capital letters and with different superscript capital letters mean no significant difference () and significant difference (), respectively.
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