Research Article
Resveratrol Attenuates Oxidative Stress-Induced Intestinal Barrier Injury through PI3K/Akt-Mediated Nrf2 Signaling Pathway
Figure 7
Effects of resveratrol on H2O2-induced cytotoxicity in Nrf2-knockdown IPEC-J2 cells. The Nrf2-knockdown (shNRF2-IPEC-J2) or control (SC-IPEC-J2) cells were pretreated with or without 50 μM RSV for 6 hours and then cocultured with 500 μM H2O2 for 4 hours. (a) Cell viability was measured using the CCK-8 assay. Results are presented as the percentage of cell viability compared with the control (0 μM). (b, c) The relative expression of SOD-1, CAT, claudin-1, occludin, and ZO-1 was detected by qRT-PCR. Data are shown as ratios of abundance of target gene transcripts in the treated cells to those in the control cells after normalization to β-actin. (d) Apoptotic cells were analyzed by flow cytometry using Annexin V-PI double staining. (e) Protein levels of Nrf2, Akt, p-Nrf2, and p-Akt were detected by Western blot with β-actin as the loading control. Values are the . The columns with the same superscript capital letters and with different superscript capital letters mean no significant difference () and significant difference (), respectively.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |