Toxic Acetaminophen Exposure Induces Distal Lung ER Stress, Proinflammatory Signaling, and Emphysematous Changes in the Adult Murine Lung

Figure 2

Toxic APAP exposure induces proximal and distal lung injury. Representative H&E-stained (a) proximal control (A) and APAP-exposed (B) (24 hours, 280 mg/kg, IP) adult male ICR mice and (b) distal control (C) and APAP-exposed (D) (24 hours, 280 mg/kg, IP) adult male ICR mice. Red arrows indicate epithelial cells in the airway lumen. Yellow arrows indicate distal lung macrophages. Yellow circles indicate emphysematous-like changes of the breakdown of alveolar walls and the “clubbing” of the broken wall tops. (c) Respiratory/terminal bronchiole epithelial injury, (d) bronchus-associated lymphoid tissue (BALT), (e) peripheral lung emphysema score, (f) peripheral lung macrophage load, (g) surface area, (h) mean linear intercept, and (i) airspace area in control vs. APAP-exposed (280 mg/kg, IP) adult male ICR mouse lung. per time point. Data are expressed as ; vs. unexposed control.

(a) Proximal Lung

(b) Distal Lung

(c)

(d)

(e)

(f)

(g)

(h)

(i)