Toxic Acetaminophen Exposure Induces Distal Lung ER Stress, Proinflammatory Signaling, and Emphysematous Changes in the Adult Murine Lung

Figure 6

Toxic APAP exposure induces proinflammatory NFκB signaling in the distal lung. (a) Representative Western blot and densitometric analysis of NFκΒ inhibitory proteins p105, IκBα, and IκBβ in distal lung cytosolic extracts after APAP exposure (2, 4, and 8 hours; 280 mg/kg, IP). GAPDH is shown as loading control. Data is expressed as ( per time point). vs. unexposed control. (b) Representative Western blot and densitometric analysis of NFκΒ subunits p65 and p50 in distal lung nuclear extracts after APAP exposure (2, 4, and 8 hours; 280 mg/kg, IP). HDAC is shown as a loading control. GAPDH is shown to confirm the purity of the nuclear sample. Data is expressed as ( per time point). vs. unexposed control. (c) Fold change in NFκΒ target gene mRNA expression: Mmp9, Ccl2, Il1b, Nfkbia, Gclc, and Ptgs2 following APAP exposure (8 hours; 280 mg/kg, IP). Data expressed as ( per time point). 5 vs. unexposed control.

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