Naringenin Attenuates Myocardial Ischemia-Reperfusion Injury via cGMP-PKGI<i>α</i> Signaling and In Vivo and In Vitro Studies

<div>Evaluation of myocardial oxidative stress markers and cGMP-PKGI<i>α</i> signaling. All the measurements were carried out after 4 hours of simulated reperfusion. (a) Cellular superoxide generation, (b) cellular malondialdehyde (MDA) contents, (c) representative blots, (d) gp91<i><sup>phox</sup></i> expression, (e) catalase expression, (f) MnSOD expression, (g) cellular cGMP level, (h) representative blots, (i) PKGI<i>α</i> expression, and (j) VASP (Ser239) phosphorylation. Data are expressed as <span class="nowrap"><svg height="9.11633pt" id="M41" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.7664 67.6442 9.11633" width="67.6442pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.595,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 374 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557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg><i> vs.</i> SIR group, and <svg height="12.475pt" id="M46" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -11.838 51.9505 12.475" width="51.9505pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M724 428H489V396C558 388 570 377 552 332C533 288 517 255 494 220C431 283 362 343 329 370C366 395 388 414 407 430C430 449 449 478 449 518C449 598 382 657 300 657C209 657 133 590 133 499C133 444 161 403 204 363C116 299 54 261 54 169C54 81 127 -14 255 -14C327 -14 396 20 468 116C500 84 539 36 553 22C565 8 621 -14 677 -19L684 -8C652 49 565 147 521 191C557 244 580 284 607 323C640 373 654 388 724 396V428ZM288 619C337 619 373 573 373 516C373 465 347 427 300 392C236 437 207 471 207 522C207 579 241 619 288 619ZM282 57C214 57 142 105 142 197C142 246 168 291 233 338C329 262 390 203 441 146C409 101 349 57 282 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602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,42.885,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,45.849,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,52.089,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg><i> vs.</i> SIR + NRG group. SIR: simulated myocardial ischemia-reperfusion; NRG: naringenin.</div>

Oxidative Medicine and Cellular Longevity

fig7

Figure 7

Figure 7: Naringenin Attenuates Myocardial Ischemia-Reperfusion Injury via cGMP-PKGI<i>α</i> Signaling and In Vivo and In Vitro Studies 

Oxidative Medicine and Cellular Longevity

Naringenin Attenuates Myocardial Ischemia-Reperfusion Injury via cGMP-PKGIα Signaling and In Vivo and In Vitro Studies

Figure 7