Nanoencapsulated Quercetin Improves Cardioprotection during Hypoxia-Reoxygenation Injury through Preservation of Mitochondrial Function

Figure 4

NP internalization into H9c2 cells. Representative fluorescence images, acquired by laser scanning confocal microscopy, of the incorporation of PLGA-FITC NP in H9C2 cells after 24 h of incubation. Staining of cells is as follows: blue: DRAQ5-stained nuclei; red: Alexa Fluor 568-conjugated phalloidin-stained actin filaments; and green: FITC-labeled PLGA NPs. (a) Representative projection of an optical section of the cells. (b, c) Orthogonal projections and , reconstructed from the optical section stacks, acquired with 1 μm of distance between each stack. Scale bars: 25 μm.