Alterations in Organismal Physiology, Impaired Stress Resistance, and Accelerated Aging in Drosophila Flies Adapted to Multigenerational Proteome Instability
G80-BTZ flies were mildly resistant to BTZ. (a) Relative (%) CT-L and C-L proteasome activities in somatic tissues of NT-BTZ and G80-BTZ flies (see Fig. S1) following exposure to 1 μΜ ΒΤZ for 4 days (4D), 10 days (10D), or 20 days (20D). (b) Representative immunoblotting analysis of ubiquitin (Ub) levels in somatic tissue lysates of shown young flies’ groups (compared to control samples from NT flies shown in Fig. S2B1); GAPDH probing was used as input reference. (c) Stereoscopic images of 3rd instar larvae and late-stage pupae of indicated groups. (d) Longevity curves of female and male NT-BTZ and G80-BTZ flies exposed to 1 μΜ ΒΤZ. Bars, ±SD ()..
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