Alterations in Organismal Physiology, Impaired Stress Resistance, and Accelerated Aging in Drosophila Flies Adapted to Multigenerational Proteome Instability
Sustained proteome instability caused metabolic reprogramming in G80-BTZ flies being evidenced by altered mitochondrial biogenesis. (a) CLSM visualization following immunofluorescence staining of young flies’ thoracic muscle tissues with ATP5a antibody; samples were counterstained with DAPI. (b) Relative expression levels of mitochondrial biogenesis (PGC-1, TFAM) and chaperones (Hsp10, Hp60A, and Hsc70-5) genes in shown young flies’ somatic tissues. (c1) Relative expression levels of mitochondrial energetics (SdhA, ATPsynβ), quality control (Lon), dynamics (Marf, Opa1, and Drp1), and mitophagy (park, Pink1) genes in indicated young flies’ somatic tissues. (c2) ATP5a expression levels (blue native-PAGE; upper panel) and relative (%) quantitation (lower panel) in isolated mitochondria from somatic tissues of young female and male flies; GAPDH probing in cytosolic preparations was used as an input reference. (d) Mitochondrial ST3/ST4 respiratory ratio in somatic tissues of the indicated flies’ groups. Gene expression was plotted vs. the respective control, and Rp49 gene expression was used as input reference. Bars, ±SD ().;.
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