Neuroprotective Effect of SCM-198 through Stabilizing Endothelial Cell Function

Figure 3

SCM-198 improved bEnd.3 cell antioxidative capacity in vitro. SCM-198 exhibited antioxidant abilities and improved the cell viability of bEnd.3. (a) Cell viability, evaluated by an MTT assay, was significantly reduced after OGD/R injury exposure, while 1 μM and 10 μM of SCM-198 could increase cell viability. (b) MDA level of the SCM-198 group was remarkably decreased as compared to the OGD/R group. (c) SCM-198 could predominantly increase intercellular antioxidative capacity by restoring the CAT activity. (d) SCM-198 obviously improved the expression of SOD1. (e) SCM-198 reduced the cell apoptosis in bEnd.3. OGD/R-induced cell apoptosis was determined by TUNEL staining. (f) The result showed that OGD/R obviously increased the apoptosis ratio, whereas treatment with SCM-198 reduced cell apoptosis. Values are expressed as . versus control group, versus OGD group ().

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