Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response

Figure 3

Treatment with MRS inhibited the expression of inflammatory cytokines at the mRNA level in acetic acid-induced UC. Mice were administered with MRS at a dose of 1 or 10 ml/kg by gastric gavage for a week before colitis establishment. SASP was used as a positive control at a dose of 500 mg/kg. Colitis was induced by acetic acid solution () injection into the lumen of the colon intrarectally with a volume of 1 ml and dose of 5% except for the sham group. Total RNA was collected from colon specimens, and the expression levels of (a) TNF-α, (b) IL-6, (c) IL-1β, and (d) IL-10 were tested. Data were expressed as . , , and .

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