Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response
Treatment with MRS activated the anti-inflammatory response-related IL-10/JAK1/STAT3 signaling pathway. Mice were administered with MRS at a dose of 10 ml/kg by gastric gavage for a week before colitis establishment. SASP was used as a positive control at a dose of 500 mg/kg. Colitis was induced by acetic acid solution () injection into the lumen of the colon intrarectally with a volume of 1 ml and dose of 5% except for the sham group. Colon tissues were collected, and the protein levels of IL-10, p-JAK1, JAK1, p-STAT3, and STAT3 were analyzed by Western blotting. Data were expressed as . and .