Thioredoxin Reductase-1 Inhibition Augments Endogenous Glutathione-Dependent Antioxidant Responses in Experimental Bronchopulmonary Dysplasia

Figure 5

Total GSH levels and GPx activity in murine transformed club cells. mtCCs were treated with 0.5 μM AFN (or vehicle, DMSO) for up to 24 h. Total GSH was measured at 1, 6, and 24 hr in the lysate (a) and at 6 and 24 hr in media (b). Data (-9) were analyzed by one-way ANOVA. In separate studies, mtCCs were treated with DMSO or 0.5 μM AFN in DMSO in the presence or absence of buthionine sulfoximine (BSO). vs. 1 h control; vs. 6 h control; vs. 24 h control. Total GSH levels in lysate (c) and media (d) at 24 hr were determined. GPX2 expression (e) and total GPx activity (f) were also determined. Expression data is representative of 5-6 samples from two independent experiments, and activity data is per one independent experiment. Data (-6) were analyzed by two-way ANOVA followed by Tukey’s post hoc analysis. vs. vehicle; vs. AFN; vs. BSO.

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