The Sirt1/P53 Axis in Diabetic Intervertebral Disc Degeneration Pathogenesis and Therapeutics

Figure 1

High glucose suppresses cell viability and promotes apoptosis in nucleus pulposus cells in vitro. (a) The cytotoxicity of glucose in NP cells was determined at different concentrations at a duration of 24 h using the CCK-8 assay. (b) The cytotoxicity of glucose in the nucleus pulposus was determined via the CCK-8 assay using a glucose concentration of 50 μM for different durations. (c–d) The TUNEL assay was performed on the NP cells treated with different concentrations of glucose (original magnification ×200, scale bar: 50 μm) and used in the quantification of apoptotic-positive cells. (e, g) The expression of cleaved caspase-3, Bax, and Bcl-2 evaluated by western blot in NP cells treated with glucose in a dose-dependent manner and a time-dependent manner. (f, h) The quantification of Bcl-2, Bax, and cleaved caspase-3 immunoblots. The experiment was repeated at least three times, with a representative example shown. The data in the figures represent the . Significant differences between groups are indicated as , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)