The Sirt1/P53 Axis in Diabetic Intervertebral Disc Degeneration Pathogenesis and Therapeutics

<div>Butein treatment eliminates ROS in nucleus pulposus cells via the Sirt1 pathway. (a) ROS were measured in NP cells using an ROS assay kit (original magnification ×400, scale bar: 25 <i>μ</i>m). (b) The expression of Sirt1, ace-p53, and p53 was evaluated in NP cells by western blot, and representative results of the western blots are presented. (c, d) The quantification of Sirt1, ace-p53, and p53 immunoblots is shown. The experiment was repeated three times independently, with a representative example shown. 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Oxidative Medicine and Cellular Longevity

fig10

Figure 10

Figure 10: The Sirt1/P53 Axis in Diabetic Intervertebral Disc Degeneration Pathogenesis and Therapeutics 