SYCP3 was expressed in germ cells of both male and female Wt1R394W/R394W gonads at E13.5. SYCP3/MVH double-staining experiment was performed with control Wt1+/R394W (A, B) and Wt1R394W/R394W (C, D) embryos at E13.5. Germ cells were labeled with MVH (green). DAPI (blue) was used to stain the nuclei. The arrowheads point to SYCP3-positive germ cells in Wt1R394W/R394W gonads. The gender of the embryos was confirmed with PCR using Sry primers.