Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 5

Research Article

Differential Modulation of NF-κB in Neurons and Astrocytes Underlies Neuroprotection and Antigliosis Activity of Natural Antioxidant Molecules

Figure 5

Effect of antioxidants on neuronal NF-κB binding activity. (a–d) EMSA of nuclear extracts prepared from cortical neurons treated for 2 h with CM from astrocytes stimulated with TNFα (a–c) or LPS (d). Where indicated, neurons were pretreated for 30 min with RSV (10 μM) (a), QRC (10 μM) or LYC (10 μM) (b), ALA (10 μM) or NAC (300 μM) (c), or the pool (a, d). (e) Competition assay with excess unlabeled oligonucleotide on nuclear extracts from neurons treated with TNFα or HeLa cells (positive CTR, EMSA NF-κB kit, Panomics). (f) Western blot analysis of p65 protein in cytosol or nuclear extracts from neurons treated with CM-LPS. (g) Quantitative analysis of NF-κB binding activity by ImageJ software. Data, expressed as percent of CTR, are the of three experiments. versus CTR (ANOVA and Dunnett’s multiple comparison test).

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