Pueraria lobata and Daidzein Reduce Cytotoxicity by Enhancing Ubiquitin-Proteasome System Function in SCA3-iPSC-Derived Neurons

Figure 2

Differentiation of neural progenitors and neurons from control- and SCA3-iPSCs. (a) Flow chart of neuronal differentiation from iPSCs. KO: Knockout medium; SB: SB431542. (b) Neural stem cells (NSCs) positive to NESTIN (green) and PAX6 (red) were generated following three weeks of differentiation from iPSCs. (c) Matured neurons expressing TUJ1 (red) were obtained following another two weeks of differentiation from NSCs. Scale bar: 50 μm. (d) Cell viability was determined by the trypan blue exclusion assay, and (e) caspase 3 activity was examined in the control- (NC-) and SCA3-iPSC-derived neurons. The caspase 3 activity in NC-iPSC-derived (NC-1 and NC-2) neurons was normalized as 100%. Each experiment for each sample was performed in triplicate.

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