Research Article

Pueraria lobata and Daidzein Reduce Cytotoxicity by Enhancing Ubiquitin-Proteasome System Function in SCA3-iPSC-Derived Neurons

Figure 7

NH037 and daidzein reduced cytotoxicity and caspase 3 expression/activity in MG132-treated SCA3-iPSC-derived neurons. (a) LDH assay in iPSC-derived neurons treated with 2 μM MG132 alone or MG132 and 1 mg/ml NH037/50 μM daidzein (untreated neurons were normalized as 100%). (b) Western blot analysis of cleaved caspase 3 (normalized to TUJ1 as the internal control) in iPSC-derived neurons treated with 2 μM MG132 alone or MG132 and 1 mg/ml NH037/50 μM daidzein. The levels of cleaved caspase 3 from MG132-treated NC-iPSC-derived neurons (NC-1 and NC-2) were set as 100%. (c) Caspase 3 activity in iPSC-derived neurons treated with 2 μM MG132 alone or MG132 and 1 mg/ml NH037/50 μM daidzein. Untreated neurons were normalized as 100%. Each experiment for each sample was performed in triplicate. values: MG132-treated vs. untreated, , , and ; SCA3-1 vs. NC-1 or SCA3-2 vs. NC-2, &, &&, and &&&; MG132/NH037 vs. MG132 alone or MG132/daidzein vs. MG132 alone, #, ##.
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