Curcumin Suppresses Hepatic Stellate Cell-Induced Hepatocarcinoma Angiogenesis and Invasion through Downregulating CTGF

Figure 2

Curcumin decreased VEGF, IL-6, and SDF-1 expression in HCC via inhibiting HIF-1α. St Med stands for standard media of PSC cells, CM stands for conditioned media from HepG2 cells, CM + Cur stands for conditioned media from HepG2 cells pretreated with curcumin, CM + NAC stands for conditioned media from HepG2 cells pretreated with NAC, sh-HIF-1α-CM + Cur stands for HSC cell knockdown with sh-HIF-1α and cultured with conditioned media from HepG2 cells pretreated with curcumin, sh-HIF-1α-CM + NAC stands for HSC knockdown with sh-HIF-1α and cultured with conditioned media from HepG2 cells pretreated with NAC, and sh-HIF-1α-CM stands for HSC cell knockdown with sh-HIF-1α and cultured with conditioned media from HepG2 cells. ELISA was assayed to assess IL-6 (a), VEGF (b), and SDF-1 (c) expression in the conditioned medium of the indicated groups. IL-6 (d), VEGF (e), and SDF-1 (f) mRNA expression in HSCs was detected by qRT-PCR, as described in the Materials and Methods. versus St Med group (), versus CM (). All data are representative of at least three independent experiments.

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