Curcumin Suppresses Hepatic Stellate Cell-Induced Hepatocarcinoma Angiogenesis and Invasion through Downregulating CTGF

Figure 3

Curcumin abrogated HSC-induced increases in HIF-1α, MMP-9 expression and EMT process in HepG2 cells though down-regulating HIF-1α. St Med stands for standard media of HepG2 cells, CM stands for conditioned media from HSCs, CM + Cur stands for conditioned media from HSCs with curcumin exposure, CM + NAC stands for conditioned media from HSCs with NAC exposure, sh-HIF-1α-CM + Cur stands for sh-HIF-1α knockdown HepG2 cells treated with conditioned media from HSCs pretreated with curcumin, sh-HIF-1α-CM + NAC stands for sh-HIF-1α knockdown HepG2 cells treated with conditioned media from HSCs pretreated with NAC, and sh-HIF-1α-CM stands for sh-HIF-1α knockdown HepG2 cells treated with conditioned media from HSCs. (A&B&C) HIF-1α, MMP-9, E-cadherin, vimentin, and β-actin protein expression levels were evaluated by immunoblotting. versus St Med group (), versus CM group (). (d, e) HIF-1α, MMP-9, E-cadherin, vimentin, and β-actin mRNA expression levels were determined by qRT-PCR. versus St Med group (); versus CM (). All data are representative of at least three independent experiments.

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