Curcumin Suppresses Hepatic Stellate Cell-Induced Hepatocarcinoma Angiogenesis and Invasion through Downregulating CTGF

Figure 4

Curcumin suppressed HSC-induced invasion in HepG2 cells through decreasing HIF-1α. St Med stands for standard media of HepG2 cells, CM stands for conditioned media from HSCs, CM + Cur stands for conditioned media from HSCs with curcumin exposure, CM + NAC stands for conditioned media from HSCs with NAC exposure, sh-HIF-1α-CM + Cur stands for sh-HIF-1α knockdown HepG2 cells treated with conditioned media from HSCs pretreated with curcumin, sh-HIF-1α-CM + NAC stands for sh-HIF-1α knockdown HepG2 cells treated with conditioned media from HSCs pretreated with NAC, and sh-HIF-1α-CM stands for sh-HIF-1α knockdown HepG2 cells treated with conditioned media from HSCs. The cells were placed in a Matrigel-coated invasion chamber for 20 h. (a, b) We evaluated invasion ability by counting the numbers of migrated cells in ten randomly selected fields under a light microscope at ×100 magnification. versus St Med group (), versus CM (). All data are representative of at least three independent experiments.

(a)

(b)