Decreased Nuclear Ascorbate Accumulation Accompanied with Altered Genomic Methylation Pattern in Fibroblasts from Arterial Tortuosity Syndrome Patients

Figure 1

Subcellular distribution of ascorbate in human fibroblasts. Subcellular distribution of ascorbate in human fibroblasts derived from healthy controls () was assessed by immunoelectron microscopy. After pretreatment of ascorbate or dehydroascorbic acid, cells were fixed and labeled with an antibody raised against ascorbate and analyzed following immunogold staining. (a) A representative image of the electron microscopy is shown. Scale bar is 1 μm. N: nucleus, Nu: nucleolus. (b) Distribution of gold particles—representing ascorbate—was determined with respect to the relative area of the cytosol or the nucleus. A significantly higher level of the vitamin was observed in the nucleus compared to cytosol after both ascorbate and dehydroascorbate treatment. Data are , , .

(a)

(b)