Decreased Nuclear Ascorbate Accumulation Accompanied with Altered Genomic Methylation Pattern in Fibroblasts from Arterial Tortuosity Syndrome Patients

<div>Gene region-specific cytosine modifications in peroxisome proliferator-activated receptor gamma gene using differential restriction endonuclease cleavage. The EpiMark 5-hmC and 5-mC Analysis Kit uses a glycosylation pretreatment to distinguish 5-hmC from 5-mC via differential restriction endonuclease digestion of a CCGG sequence. The relative ratios (percentage of 5-mC, 5-hmC, and non-modified C) are quantified using quantitative PCR amplification. Average <svg height="9.58608pt" id="M23" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -9.23615 71.076 9.58608" width="71.076pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M478 437H300V411C357 404 363 393 347 345C325 280 293 190 258 107C229 178 188 284 163 354C147 397 149 405 203 411V437H-2V411C52 403 59 396 82 339C130 222 176 109 220 -11H248C304 138 356 261 390 336C417 395 425 404 478 411V437Z"></path></g><g transform="matrix(.013,0,0,-0.013,6.215,0)"><path d="M433 39L423 65C413 59 399 54 387 54C370 54 352 69 352 114V299C352 352 342 392 307 422C285 440 255 449 225 449C168 437 102 399 75 379C56 365 44 353 44 339C44 315 69 296 87 296C101 296 111 303 116 319C124 349 133 371 145 385C156 397 171 404 190 404C241 404 275 364 275 291V274C253 256 180 229 120 209C65 190 39 159 39 110C39 47 88 -12 159 -12C189 -12 237 25 277 52C282 35 288 21 301 8C312 -3 333 -12 348 -12L433 39ZM275 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Decreased Nuclear Ascorbate Accumulation Accompanied with Altered Genomic Methylation Pattern in Fibroblasts from Arterial Tortuosity Syndrome Patients 

Figure 5 | Decreased Nuclear Ascorbate Accumulation Accompanied with Altered Genomic Methylation Pattern in Fibroblasts from Arterial Tortuosity Syndrome Patients 