lncRNA AK054386 Functions as a ceRNA to Sequester miR-199 and Induce Sustained Endoplasmic Reticulum Stress in Hepatic Reperfusion Injury

Figure 1

miR-199 protects hepatocytes from IRI in vitro and in vivo. The relative levels of miR-199a-5p in mouse liver tissues from the control, ischemia, and IRI groups. RNA levels were quantified by qRT-PCR and normalized to U6. Five mice were analyzed in each group. ANOVA, . (b) The relative levels of ER stress-related genes as measured by qRT-PCR and normalized to GAPDH. ANOVA, . (c) miR-199 impacts BNL-CL2 apoptosis in an IRI cell model. Apoptosis rates were assayed by flow cytometry. (d) BNL-CL2 cell death levels were assayed by LDH release. (e) Mouse serum ALT and AST levels were analyzed and presented as the (). ANOVA, . (f) Representative light photomicrographs of TUNEL-stained sections of mouse liver tissue from control mice and mouse hepatic IRI models. Five mice were analyzed in each group. (g, h) Expression of ER stress-related genes, as measured by (g) qRT-PCR and (h) Western blot in the mouse BNL-CL2 cell line. The qRT-PCR results were normalized to GAPDH. Con and BNL-CL2 cells were cultured under normal conditions, and IRI and BNL-CL2 cells were cultured under IRI conditions. Data are shown as the of 5 independent experiments. ANOVA, and .

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