Research Article | Open Access
L. Dalle Carbonare, M. Mottes, S. Cheri, M. Deiana, F. Zamboni, D. Gabbiani, F. Schena, G. L. Salvagno, G. Lippi, M. T. Valenti, "Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity", Oxidative Medicine and Cellular Longevity, vol. 2019, Article ID 8426259, 14 pages, 2019. https://doi.org/10.1155/2019/8426259
Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity
Lack of physical exercise is considered an important risk factor for chronic diseases. On the contrary, physical exercise reduces the morbidity rates of obesity, diabetes, bone disease, and hypertension. In order to gain novel molecular and cellular clues, we analyzed the effects of physical exercise on differentiation of mesenchymal circulating progenitor cells (M-CPCs) obtained from runners. We also investigated autophagy and telomerase-related gene expression to evaluate the involvement of specific cellular functions in the differentiation process. We performed cellular and molecular analyses in M-CPCs, obtained by a depletion method, of 22 subjects before (PRE RUN) and after (POST RUN) a half marathon performance. In order to prove our findings, we performed also in vitro analyses by testing the effects of runners’ sera on a human bone marrow-derived mesenchymal stem (hBM-MSC) cell line. PCR array analyses of PRE RUN versus POST RUN M-CPC total RNAs put in evidence several genes which appeared to be modulated by physical activity. Our results showed that physical exercise promotes differentiation. Osteogenesis-related genes as RUNX2, MSX1, and SPP1 appeared to be upregulated after the run; data showed also increased levels of BMP2 and BMP6 expressions. SOX9, COL2A1, and COMP gene enhanced expression suggested the induction of chondrocytic differentiation as well. The expression of telomerase-associated genes and of two autophagy-related genes, ATG3 and ULK1, was also affected and correlated positively with MSC differentiation. These data highlight an attractive cellular scenario, outlining the role of autophagic response to physical exercise and suggesting new insights into the benefits of physical exercise in counteracting chronic degenerative conditions.
Lack of physical exercise is considered an important risk factor for chronic diseases. On the contrary, physical exercise counteracts obesity, diabetes, bone disease, and hypertension  as well as other degenerative diseases [2, 3]. In addition, physical exercise plays a central role in maintaining bone quality . Recently, we identified several proteins differentially expressed in runners’ sera after the half marathon. Despite several negative cellular effects, such as oxidative stress, the resulting proteome modulation seems to be beneficial for the overall health . We also demonstrated that running the half marathon increases the expression of the osteogenic transcription factor RUNX2 as well as the calcification process in an in vitro model. Furthermore, we found a reduction in the adipogenic commitment of a human bone marrow-derived mesenchymal stem cell (hBM-MSC) line treated with sera obtained from runners . MSCs, together with hematopoietic, endothelial, and smooth or skeletal muscle precursors can be found in peripheral blood as circulating progenitor cells (CPCs) [6, 7]. In the past, we have described the isolation of MSC-like cells from peripheral blood (CD3-, CD19-, CD14low, CD45low, and CD34low), characterized by clonogenic growth and differentiation ability . In addition, these mesenchymal circulating progenitor cells (M-CPCs) expressed also CD105 and CD73 markers ; we have investigated their involvement in various skeletal degenerative diseases [9–12]. Considering the clinical and social impact of common skeletal degenerative diseases such as osteoporosis and ostearthrosis, we were interested to evaluate the effects of physical activity on M-CPCs. Since during physical activity oxidative stress as well as pleiotropic cytokine production increases, we hypothesized that different biological processes involved in differentiation might be affected in progenitor cells. In particular, telomerase activity and autophagy, which are both involved in oxidative stress [13–15], have been suggested as biological processes affecting differentiation [16, 17]. Therefore, we hypothesized that physical activity might promote differentiation by regulating telomerase-associated genes as well autophagy.
Several studies have demonstrated that the progenitor cell number increases in peripheral blood in response to endurance and physical exercise [18–21]. Various kinds of exercise such as running, rowing, and cycling may stimulate progenitor cell mobilization; stimulation of stem cell formation sites, such as the pelvis and femur, can be considered responsible for this [19, 22]. Inflammation-related [5, 23] or adrenaline-induced factors  might affect progenitor cell mobilization during exercise. However, the proliferative potential of MSCs declines with age because of telomere shortening [25, 26]. In addition, telomeres can be affected by genotoxic injuries and oxidative stress . Notably, short telomeres affect the proliferation, self-renewal, and commitment of progenitor cells  and induce alterations during their differentiation process in embryonic stem cells . However, while telomere length is important to counteract mesenchymal stem cell senescence, , telomerase activity plays an important role in the differentiation process . Increased telomerase activity due to the overexpression of human telomerase reverse transcriptase (hTERT) gene in hMSCs promotes osteoblast differentiation in vitro as well as heterotopic bone formation in vivo [31, 32]. Besides maintaining telomere length, hTERT increases stem cell proliferation, rescues the impaired differentiation, and, in this way, counteracts senescence-induced negative effects . Senescence may be spurred by autophagy deregulation leading to intracellular waste accumulation . Autophagy plays an important role in differentiation and stemness regulation of various cellular types, including MSCs . Its activation has been suggested to counteract bone loss  as well as MSC senescence . Autophagy also regulates adipocyte differentiation and lipid accumulation and plays an important role in the balance between white and brown fat . Conversely, impaired autophagy in MSCs can induce metabolic diseases [35, 37–39]. Thus, autophagy is considered an “antiaging dynamic system” because of its contribution to cell renewal and homeostasis . It has been reported that telomerase regulates autophagy [41, 42]; an increase in telomerase activity and autophagy has been demonstrated following calorie restriction .
The aim of this study was to understand whether physical activity may promote osteogenic differentiation of CPCs. We investigated the involvement of cellular processes such as autophagy and telomere maintenance in osteogenic differentiation by means of PCR array analysis.
Therefore, in order to better understand the effects of physical activity on M-CPCs, we investigated their commitment as well as their modulation of telomerase-related genes, following the half marathon. We evaluated the effects of half marathon also on the autophagic process, as a supposed mechanism influencing M-CPC differentiation. In addition, we performed in vitro experiments supporting our findings.
2. Materials and Methods
22 male runners and 10 age-matched male controls were included in the study (Figure 1). Study subjects were healthy and regularly active. Participants were enrolled during sport events called “run for science,” held in Verona (Italy) in April 2016 and April 2017. Twenty-two male runners (median age: ; median BMI: 22.6) carried out a 21.1 km half marathon in hours. Participants were all recreational runners and physically fit, with a median training regimen in the last 10 years: 220 minute/week and with an estimated mean VO2 max of 44.9 at the end of the run, classified as great for males with similar mean age . Controls have median age of , median BMI of 24.2, and estimated VO2 of 36-41 [45, 46] and had not been training for years. All runners and controls underwent a clinical evaluation and a medical history interview in order to exclude comorbidities or drug intake.
Blood was collected from controls and runners at the same time of day. Blood samples, obtained by venipuncture, were collected immediately before and 15 min after the run, respectively. Written informed consent was obtained from all participants, and the study was approved by the ethical committee of Azienda Ospedaliera Universitaria Integrata of Verona, Italy (number 1538; Dec. 3, 2012; local ethical committee of Azienda Ospedaliera Integrata di Verona). The study design and methods comply with the Declaration of Helsinki. Biochemical analyses were performed before and after the run: s-calcium (Ca), s-phosphate (P), carboxy-terminal telopeptide of type I collagen (CTX), ferritin, and chlorine (Cl) were monitored; 25-hydroxy vitamin D (vitD) was also evaluated in order to exclude vitD-deficient individuals.
2.2. Serum Collection
In order to evaluate the effects of sera in vitro, we collected PRE and POST RUN sera from 10 ml of fresh blood by centrifugation at 400×g. Sera were harvested and frozen in aliquots at −80°C until use. To perform the in vitro experiments, individual PRE RUN sera and POST RUN sera, respectively, were pooled.
2.3. Circulating Progenitor Cells (CPCs)
To evaluate differentiation ability and modulation of telomerase-related genes, we isolated M-CPCs from heparinized blood as previously reported . In particular, M-CPCs were collected from 50 ml of heparinized blood by a depletion method, performed by two Ficoll procedures in order to remove hematopoietic cells using the antibody cocktail. Initially, peripheral blood mononuclear cells (PBMCs) were isolated by a gradient centrifugation at 800×g for 30 min at 20°C (first Ficoll procedure). Then, to remove the unwanted hematopoietic cells, a RosetteSep antibody cocktail (Stemcell Technologies Inc., Vancouver, Canada) was used with 5 ml of whole blood mixed with the PBMCs obtained by the first Ficoll. The antibody cocktail (against CD3, CD14, CD19, CD38, and CD66b-positive cells), at dilution of 50 μl/ml, was incubated with samples for 20 min at room temperature. Finally, a second Ficoll procedure was performed to remove the unwanted cells linked to antibody cocktail and crosslinked to red blood cells (glycophorin A). Collected cells were then washed in phosphate-buffered saline (PBS). Phenotype analysis was performed as previously described .
2.4. Analysis of Cell Phenotype
We evaluated the phenotype of M-CPCs by analyzing the gene expression for CD3, CD14, CD19, CD45, CD34, CD73, and CD105 markers, as reported previously . This method allows the analysis of cell phenotype when stringent purification strategies are performed .
2.5. In Vitro Osteogenic and Adipogenic Differentiation
Sera, obtained from each runner before and after the competition, were mixed in two pools named PRE RUN and POST RUN sera, respectively. In order to study the induction of osteogenic and adipogenic differentiation, respectively, before and after physical exercise, we treated the BM-hMSC cell line (human bone marrow-human mesenchymal stem cells, PromoCell, Heidelberg, Germany) with pooled PRE RUN and POST RUN sera .
Pooled sera were added to the above cell line at 10% final concentration. Cells were plated at a density of cells per well into 24-well plates and cultured up to 21 days. In particular, osteogenic differentiation was performed with osteogenic medium containing osteogenic stimulatory supplements (15%, Stemcell Technologies Inc., Vancouver, Canada), 10−8 M dexamethasone, 3.5 mM β-glycerophosphate, and 50 μg/ml ascorbic acid (Stemcell Technologies Inc.). The adipogenic differentiation was performed by using 0.5 mM isobutylmethylxanthine, 200 μM indomethacin, 10−6 M dexamethasone, and 10 μg/ml insulin in basal medium. Chondrogenic differentiation was performed by culturing hMSCs with mesenchymal stem cell chondrogenic differentiation medium (PromoCell, Heidelberg, Germany). For osteogenic, adipogenic, or chondrogenic differentiation, the medium was changed every 3 days after initial plating.
2.6. Total RNA Extraction and Reverse Transcription
Pellets from the differentiated BM-hMSC cell line treated with pooled sera and from CPCs were collected and stored at -80°C. Then, they were processed for RNA extraction with a “RNeasy® protect mini kit” by Qiagen, Hilden, Germany, following the manufacturer’s protocol. RNA samples were quantified by a Qubit™ 3 fluorometer using a “Qubit™ RNA HS assay kit” (Invitrogen, Carlsbad, USA). Two micrograms of the extracted RNAs was reverse transcribed with a “first strand cDNA synthesis kit” by GE Healthcare, Little Chalfont, UK, according to the producer’s instructions. RNA and cDNA samples were stored at -80°C.
2.7. PCR Array
PCR arrays were performed by using TaqMan™ array human osteogenesis (Thermo Fisher Corporation, Waltham, MA, USA, 4414096) and TaqMan™ array human telomere extension by telomerase (Thermo Fisher Corporation, Waltham, MA, USA, 4414187) according to the manufacturer’s instructions.
2.8. Real-Time RT-PCR
To validate the array profile of M-CPCs as well as to investigate gene expression modulation in M-CPCs and in in vitro experiments, we performed real-time PCR analyses as reported previously . Briefly, predesigned, gene-specific primers and probe sets for each gene (CD3, hs00174158_m1; CD14, hs02621496-s1; CD19, hs00174333_m1; CD45, hs00174541_ m1; CD34, hs00156373_m1; CD73, hs00159686_m1; CD105, hs00923996_m1; RUNX2, hs00231692_m1; OSTERIX (SP7), hs00541729_m1; COLLAGEN, TYPE I, ALPHA 2 (COL1A2), hs01028956_m1; OSTEONECTIN (SPARC), hs00234160_m1; OSTEOPONTIN (SPP1), hs00167093_m1; BMP1, hs00241807-m1; BMP3, hs00609638-m1; BMP4, hs03676628-s1; BMPR1A, hs1034913-g1; SOX9, hs01107818_m1; COMP, hs004359-m1; MSX1, hs00427183-m1; HNRNPA1, hs01656228-s1; MRE11a, hs00967437-m1; RAD50, hs00990023-m1; TERF1, hs00819517-m1; TERT, hs00972650-m1; COL2A1, hs00264051_m1; PPARG2, hs01115513_m1; B2M, hs999999_m1 (housekeeping); and GAPDH, 0802021 (housekeeping)) were obtained from assay-on-demand gene expression products (Thermo Fisher Corporation, Waltham, MA, USA). For autophagy gene expression, we used the following SYBR green primers: ATG3 FW GGAAGAATATGAAGAGAGTGG RV CTCATCATAGCCAAACAACC, ATG7 FW AGATTGTCCTAAAGCAGTTG RV CCATACATTCACTGAGGTTC, ULK1 FW TCAAAATCCTGAAGGAACTG RV ACCAGGTAGACAGAATTAGC, and β-actin FW GATGTATGAAGGCTTTGGTC RV TGTGCACTTTTATTGGTCTC. Ct values for each reaction were determined using TaqMan SDS analysis software (Applied Biosystems; Foster City, California, USA) as reported previously. To calculate relative gene expression levels between different samples, we performed the analyses by using the 2−ΔΔCT method and by using untreated MSC lines as calibrator.
Immunofluorescence analyses were performed to investigate osteogenic and adipogenic differentiation or autophagy in in vitro experiments. After 21-day culture, cells were fixed and processed according to the manufacturer’s protocols. Primary antibodies were diluted (as reported in the datasheet) in antibody dilution buffer and incubated overnight at 4°C. To evaluate osteogenic differentiation and autophagy, we used osteocalcin C8 antibody (sc74495, Santa Cruz, Dallas, Texas, USA) and LC3B (cat. #2775, Cell Signaling, Leiden, The Netherlands), respectively. Assessment of adipogenic stimuli was achieved by testing perilipin D1D8 (cat. #12589, Cell Signaling). Slides were then incubated with secondary antibodies Alexa Fluor® 594 anti-rabbit (cat. #r37117), Alexa Fluor® 488 anti-rabbit (cat. #a-11034), and goat mouse fluorescein conjugated (cat. Ap124f, Millipore, Burlington, Massachusetts, USA). Nuclear staining was performed by ProLong™ Gold Antifade Mountant with DAPI. Images were recorded using a Leica (Wetzlar, Germany) TCS SP5 AOBS inverted confocal microscope at 63x. To express data in a semiquantitative way, four different fields were measured for each sample, in three independent experiments with about 80–100 total cells.
2.10. Western Blotting
To investigate protein levels related to osteogenic, chondrogenic, and adipogenic differentiation or to autophagy in in vitro experiments, we performed Western blot analyses. Protein extraction was performed using RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol. A BCA assay (Thermo Fisher Scientific, Waltham, MA, USA) was used to determine protein concentrations. Protein samples were diluted in 4x Laemmli’s sample buffer (Bio-Rad, CA, US), heated for 5 min at 95°C, and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE), using a mini-PROTEAN® TGX™ Precast gradient 4-20% gel (Bio-Rad, CA, US), followed by transfer onto polyvinylidene difluoride (PVDF) membranes (Thermo Fisher Scientific, Waltham, MA, USA). PVDF membranes were probed with the primary and secondary antibodies reported in Table 1. Signals were detected using a chemiluminescence reagent (ECL, Millipore, Burlington, MA, USA) according to the manufacturer’s instructions. Images were acquired by a LAS4000 Digital Image Scanning System (GE Healthcare, Little Chalfont, UK). Densitometric analysis was performed by using ImageQuant software (GE Healthcare, Little Chalfont, UK), and the relative protein band intensity was normalized to β-actin.
2.11. Alkaline Phosphatase Activity
To evaluate the activity of alkaline phosphatase during osteogenic differentiation in vitro, MSCs were cultured in the presence of either PRE RUN or POST RUN sera. After 14 days of culture, cytoplasmic ALP was analyzed by using the Alkaline Phosphatase Kit 86-C (Sigma Aldrich GmbH, Munich, Germany) as previously reported . ALP activity was calculated by multiplying the (from 0 to 4) in 100 total cells according to the manufacturer’s instructions.
2.12. Alizarin Red Staining
To evaluate calcium deposition during osteogenic differentiation in vitro, we performed Alizarin red staining as previously described . Briefly, after 21 days of culture in the presence of osteogenic medium, cells were fixed with 70% ethanol, washed three times with water, and stained for 5 min with 40 mM Alizarin red S at pH 4.1. Cells were then gently rinsed with 1x phosphate-buffered saline for 15 min. The area stained with Alizarin red S was quantified in three independent experiments by using ImageJ software (NIH, Bethesda, MD, USA) as previously reported .
2.13. Oil Red O Staining
To evaluate the formation of lipid droplets during adipogenic differentiation in vitro, we performed Oil red O staining. The cells, cultured in adipogenic medium with added PRE RUN and POST RUN sera, respectively, were stained with Oil red O according to the manufacturer’s instructions. The total area of red pixels in the Oil red O-stained droplets/cell was determined by using the ImageJ analysis as previously reported . In particular, of red stained areas, measured at 40x magnification, were calculated in three different fields/slides and expressed as the percentage compared to the total area.
2.14. Alcian Blue Staining
To evaluate chondrogenic differentiation in in vitro experiments, we performed Alcian blue staining after 21 days of culture as previously reported . Briefly, the cells cultured in chondrogenic differentiation medium and with PRE or POST RUN sera were fixed with 95% methanol and then stained with 1% Alcian blue 8GX HCl overnight. Then, cell slides were washed and observed under a microscope.
2.15. Statistical Analysis
Results were expressed as the . Statistical analysis was assessed by Student’s paired -test. Differences with were considered significant. We performed also correlation analyses by Pearson’s test to measure linear dependence between the expression of a single autophagy gene and the transcription factor RUNX2 or SOX9. For in vitro data, analyses were applied to experiments carried out at least three times. Statistical analyses were performed using SPSS for Windows, version 22.0 (SPSS Inc., Chicago, IL, USA).
3.1. Biochemical Data
Results of biochemical evaluations in sera collected before (PRE RUN) and after the run (POST RUN) are reported in Table 2. None of the subjects showed hypovitaminosis D at the baseline. Electrolytes, in particular, sodium and potassium, showed significant variations due to dehydration, as well as iron serum concentration. Ferritin levels were also increased in POST RUN sera ().
3.2. Physical Exercise Promotes Mesenchymal Stem Cell Differentiation
We evaluated the progenitor cells’ phenotype as previously reported . In Table 3, we reported the expression of cluster differentiation (CD) of mesenchymal cell phenotype (CD 105 and CD 73). In order to prove the low hematopoietic contamination after depletion, we reported also values of hematopoietic cell phenotype CDs. No phenotypical difference was observed between PRE RUN- and POST RUN-selected cells. In order to evaluate if physical exercise induces mesenchymal differentiation in circulating progenitors, we analyzed the differentiation profiles in PRE RUN and POST RUN CPCs by the osteogenesis array and subsequent RT real-time PCR.
In order to validate these findings, we performed RT real-time PCR assays for several genes. We investigated the expression of SP7, the RUNX2 downstream gene, as well.
Most of the investigated genes were expressed at higher levels in POST RUN M-CPCs compared to PRE RUN M-CPCs (Figure 2(b)). The higher expression levels indicated a strong commitment of M-CPCs to osteogenic differentiation. In particular, RUNX2 expression in POST RUN was >3-fold higher compared to PRE RUN CPCs (); RUNX2 downstream genes COL1A2 (), SPARC (), SP7 (), and SPP1 () in turn confirmed the osteogenic trend, as their expression was higher too.
However, we observed lower POST RUN expression levels of BMP4 () and BMPR1a (), compared to PRE RUN levels. Ten controls were also included in the study, in order to monitor any bias due to technical procedures. In control samples obtained at time 0 and after 2 hrs, no differences were observed in gene expression evaluated by RT real-time PCR (Figure 1S).
Considering that the observed modulation in CPCs might be induced by circulating factors, we then tested the effects of sera in in vitro experiments. We analyzed the effects of PRE RUN and POST RUN sera on a hBM-MSC line. Findings from the MSC line culture supplemented with runners’ sera confirmed the data obtained in M-CPCs. In detail, gene expression profiles (after 7 days of culture for RUNX2 and after 14 days of culture for SPP1 and SPARC) (Figure 3(a)), alkaline phosphatase activity (Figure 3(b)), COL1A1 and BMP2 protein expression (Figure 3(c)), osteocalcin expression (Figure 3(d)), and calcium deposition (evaluated by Alizarin red staining) (Figure 3(e)) indicated the increased osteogenic differentiation and maturation of cells treated with POST-RUN sera.
To investigate the chondrogenic commitment in M-CPCs, we analyzed the expression of SOX9 transcription factor and of the cartilage-specific COL2A1 and COMP genes by RT real-time PCR. In particular, the higher expression of COL2A1 () as well as of COMP () in POST RUN M-CPCs confirmed the clues obtained from the chondrogenic determinant SOX9 () (Figure 4(a)). The hBM-MSC line supplemented with runners’ sera confirmed the enhanced chondrogenic differentiation when cells were cultured in the presence of POST RUN sera. The increased expression of chondrogenic genes (SOX9 after 7 days of culture; COL2A1 and COMP after 14 days of culture) (Figure 4(b)) of Aggrecan protein (Figure 4(c)) as well as stronger Alcian blue staining (Figure 4(d)) was detected in cultures supplemented with POST RUN sera.
To evaluate M-CPC adipogenic commitment, we analyzed the expression of the adipogenic transcription factor PPARG2. Data obtained by RT real-time PCR showed that the PPARG2 gene expression was higher in POST RUN M-CPCs (Figure 4(e)) (). No variations were observed in the expression of the above genes in progenitor cells from controls (Figure S2). However, when we treated the hBM-MSC line with POST RUN sera, PPARG2 gene expression levels (after 7 days of culture) were lower compared to those observed with PRE RUN serum treatment (Figure 4(f)) (). These latter data were confirmed by Oil red O staining of cells after 14 days of culture: a reduction of lipid droplets (Figure 4(g)) and a decreased number of PLIN1-positive cells (Figure 4(h)) as well as lower PLIN1 protein levels (Figure 4(i)) were observed.
We did not observe any effects related to osteogenic, adipogenic, or chondrogenic differentiation in the hBM-MSC line treated with control sera (Figure 3S).
3.3. Physical Exercise Affects the Expression of Telomerase-Associated Genes and Enhances Autophagy
We analyzed the expression of telomerase-associated genes and autophagy-related genes in order to evaluate the involvement of these cellular processes in the differentiation of circulating progenitors following physical exercise. Telomerase-associated genes, evaluated by gene array analysis in M-CPCs, were differentially expressed after physical exercise () (Figure 5(a)). Supplemental Table 2 reports in detail POST-RUN fold changes of gene expression.
Thereafter, we performed RT real-time PCR assays to validate the array data, which were confirmed as shown in Figure 5(b). Notably, both TERT and TERF1 POST RUN expression levels were higher than PRE RUN levels (), while POST RUN expression levels of several DNA repair genes (e.g., RAD50 and HNRNPA1) were lower (). No differences were observed in controls (Figure 4S).
Finally, we investigated the expression of autophagy-related genes, in order to gain information about the physical exercise-induced modulation of this vital cellular process. We analyzed ATG3, ATG7, and ULK1, in PRE and POST RUN M-CPCs. Autophagy-related genes appeared to be upregulated in POST RUN M-CPCs (Figure 6(a)) whereas no differences were found in controls (Figure 5S).
To confirm these findings, we treated a hBM-MSC line with PRE and POST RUN sera. We found that cells treated with POST RUN sera expressed lower levels of the autophagic flux marker p62 and higher ATG5 and LC3 levels, respectively, compared to cells treated with PRE RUN sera (Figures 6(b) and 6(c)).
Interestingly, by using Pearson’s correlation test, we found a positive correlation between ATG3 and SOX9 expression () and ATG3 and RUNX2 expression (). Similarly, positive correlations between ULK1 and SOX9 expression () and ULK1 and RUNX2 expression () were observed.
Our findings call for some comments on the biological effects of half marathon. Biochemical evaluations showed a significant increase of iron levels, possibly related to tissue injury, as previously suggested , while the increased ferritin levels might be attributed to the inflammatory process related to the run. However, the increased serum ferritin levels point also towards the autophagic process activation in response to physical activity. In fact, it has been demonstrated that serum ferritin is secreted from macrophages by an “atypical” vesicular system involving the autophagic process . In particular, ferritin gets into the endolysosomal system via NCOA4 autophagy receptor . Therefore, autophagy is involved in the regulation of cellular redox status modulated by physical activity , through the degradation of iron-binding proteins such as ferritin .
In this study, we also confirmed the role of physical exercise in inducing osteogenic differentiation. In particular, for the first time, we reported osteogenic gene expression modulation during physical activity. The expression of most osteogenesis-related genes, namely, RUNX2, MSX1, and SPP1, appeared upregulated after running. Array data showed increased levels of BMP2 and BMP6, according to their role in promoting osteogenesis . The in vitro effects of sera on the hBM-MSC line confirmed the enhanced osteogenic differentiation due to physical exercise. On the other hand, the observed lower expression of BMP4 in M-CPCs was intriguing: it might be explained considering that BMP4 plays a dual role both in adipogenesis and in metabolism . Consistently, we observed a downregulation of BMPR1a gene in POST RUN M-CPCs, as BMP4 signal transduction is mediated by BMPR1a receptor . In addition, it has been demonstrated that BMP4 reduces PPARG2 and increases PPARG1 expression in order to drive the terminal differentiation to white-like adipocyte at brown adipocyte’s expense . Indeed, we observed an increased expression of PPARG2 in M-CPCs. This finding is in agreement with the role of PPARG2 in regulating insulin sensitivity and glucose utilization as well as energy homeostasis . Conversely, we did not observe increased PPARG2 gene expression in the hBM-MSC line treated with POST RUN sera. In addition, the number of lipid droplets as well as PLIN1 protein levels was reduced in MSCs treated with POST RUN sera suggesting that physical activity modulates negatively adipogenic differentiation. Moreover, we observed a higher expression of BMP3 after the athletic performance. Unlike BMP2 and BMP6, BMP3 reduces osteogenesis; it also promotes preadipocyte proliferation by activating the TGFbeta/ACTIVIN-signaling pathway. Interestingly, we have previously demonstrated that physical exercise modulates miRNA expression in progenitor cells promoting osteogenesis . Otherwise, by acting on WISP1, BMP3 stimulates MSC proliferation . This finding explains our results related to the expression of both TERT and TERF1 genes which was higher in M-CPCs after the run. We assume that physical exercise may exert a dual action on progenitor cells by enhancing their proliferation and by inducing their commitment and differentiation. It is worth mentioning that hTERT plays an important role among the cellular processes affecting osteogenic differentiation [31, 32]. Murine MSCs with knock-down telomerase activity cannot differentiate into adipocytes or chondrocytes  while telomerase-overexpressing cells have an increased osteogenic ability . However, TERT expression in MSCs is debatable. It is known that telomerase activity is reduced in differentiating progenitor cells with reduced proliferative ability . Telomerase levels in MSCs are low or absent  and can be upregulated by stimuli . Interestingly, among the stimuli inducing telomerase expression, oxidative stress plays an important role . Therefore, the increased telomerase expression observed in POST RUN CPCs could be due to the oxidative stress following physical activity.
In addition, in POST RUN M-CPCs, we observed the upregulation of genes associated with chondrogenic differentiation such as SOX9, COMP, and COL2A1. The in vitro experiments confirmed these findings. These data not only demonstrate that physical exercise induces progenitor cell commitment to the chondrogenic lineage but also suggest that chondrocytic maturation is accomplished. We cannot exclude that the PRE RUN and POST RUN changes observed in gene expression result from the recruitment of different cell types. However, it has been reported that the amount of M-CPCs is not influenced by physical activity , at least immediately after the athletic performance. In fact, it has been reported that bone marrow MSC number increases following physical activity . Therefore, we can speculate that an extension of the observation interval might disclose an increased number of M-CPCs.
In M-CPCs, we observed an increased expression of genes related to autophagy, an important process involved in osteogenic differentiation . These data were confirmed in vitro. In fact, positive LC3 cells and total LC3 (LC3I+LC3II) as well as ATG5 protein expression increased while p62 decreased in MSCs treated with POST RUN sera. This finding is in agreement with our previous study where we observed, by proteomic analysis of runners’ sera, the overexpression of Von Willebrand factor (VWF) after physical exercise . Indeed, it has been demonstrated that autophagy regulates endothelial cell secretion of VWF . This evidence supports the observed elevated ferritin levels in sera after physical activity; we assume that a strong induction of autophagy may occur during an endurance trial in various cell types. Moreover, we observed a positive correlation between ATG3 and ULK1 gene expression and SOX 9 and RUNX2 gene expression in circulating progenitors. The above observations highlight the positive effect of autophagy on the chondrogenic and osteogenic differentiation, respectively; moreover, we assume that autophagy was enhanced by physical exercise as a protective mechanism in response to oxidative stress. Thus, induced autophagy promoted progenitors’ chondrogenic and osteogenic differentiation. Interestingly, it has been demonstrated that telomerase regulates the autophagic process through a glycolysis enzyme (HK2) involved in the catabolism from glucose to glucose-6-phosphate .
In summary, we suppose that the increased expression of chondrogenic and osteogenic genes in M-CPCs following exercise is due to enhanced autophagy. On the other hand, PPARG2 gene-increased expression is a consequence of insulin signaling modulation that occurs during physical activity.
Interestingly, besides the upregulation of autophagy and telomerase-associated genes, we observed downregulation of several DNA repair-related genes. This is a peculiar result since it is known that autophagy may induce either cell survival or apoptosis on the basis of the genomic damage extent . A reduced availability of proteins involved in DNA repair induces apoptosis . It is known that stem cells respond to DNA damage by activating the DNA repair systems [70, 71]. DNA repair is important for cell survival, while it may lead as well to the accumulation of genomic alterations in stem cells. Moreover, it has been shown that DNA repair activity changes from undifferentiated to differentiated hMSCs . In fact, undifferentiated adult hMSCs have higher repair activity and are resistant to radiation, while predifferentiated hMSCs may be sensitive to radiation and undergo apoptosis.
Considering this complex background, we conclude that physical exercise, e.g., half marathon, promotes autophagy in progenitor cells in response to oxidative stress, along with the differentiation process. Indeed, the upregulated TERT and TERF1 gene expression suggests active recruitment of MSCs and enhanced differentiation ability. The observed downregulation of DNA repair genes can be related to autophagy as well . Consequently, predifferentiated cells harboring genome alterations might be eliminated, promoting enhanced differentiation of progenitors after physical activity.
So far, we can conclude that half marathon physical commitment promotes osteogenic and chondrogenic differentiation as well as autophagy, through a complex biological interplay. Autophagy modulation in MSCs appears of great interest for new therapeutic approaches. In fact, autophagy in MSCs enhances their proangiogenic and immunomodulatory characteristics hence highlighting their therapeutic potential against several diseases . In this context, our findings disclose novel insights into the benefits of physical exercise in healthy and chronic degenerative conditions.
The biochemical data, array data, gene expression data, Western blot data, and staining data used to support the findings of this study are included within the article. The gene expression data of controls used to support the findings of this study and the data related to array analyses are included within the supplementary information file.
Conflicts of Interest
All authors disclose no financial and personal relationships with other people or organizations that could inappropriately influence (bias) their work.
Part of the study was carried out thanks to the support of the “Centro Piattaforme Tecnologiche” of the University of Verona, Italy. This research was funded by FUR (Fondo Unico della Ricerca; University of Verona, Italy), granted to Professor L Dalle Carbonare.
Supplemental Table 1: osteogenic Array analyses. Supplemental Table 2: telomerase related genes analyses. Figure 1S: RT real-time PCR data: expression of osteogenic genes in control samples obtained at time 0 and after 2 hrs. Figure 2S: RT real-time PCR data: expression of adipogenic genes in control samples obtained at time 0 and after 2 hrs. Figure 3S: expression of osteogenic (RUNX2), adipogenic (PPARG2), or chondrogenic (SOX9) transcription factors (A) and Alizarin red staining (B) in the MSC line treated with control sera. Figure 4S: RT real-time PCR data: expression of telomerase-related genes in control samples obtained at time 0 and after 2 hrs. Figure 5S: RT real-time PCR data: expression of autophagy-related genes in control samples obtained at time 0 and after 2 hrs. (Supplementary Materials)
- C. Bekos, M. Zimmermann, L. Unger et al., “Non-professional marathon running: RAGE axis and ST2 family changes in relation to open-window effect, inflammation and renal function,” Scientific Reports, vol. 6, no. 1, article 32315, 2016.
- S. R. Colberg, A. L. Albright, B. J. Blissmer et al., “Exercise and type 2 diabetes: American College of Sports Medicine and the American Diabetes Association: joint position statement,” Medicine & Science in Sports & Exercise, vol. 42, no. 12, pp. 2282–2303, 2010.
- J. C. Brown, K. Winters-Stone, A. Lee, and K. H. Schmitz, “Cancer, physical activity, and exercise,” Comprehensive Physiology, vol. 2, no. 4, pp. 2775–2809, 2012.
- C. Bagur-Calafat, J. Farrerons-Minguella, M. Girabent-Farres, and J. R. Serra-Grima, “The impact of high level basketball competition, calcium intake, menses, and hormone levels in adolescent bone density: a three-year follow-up,” The Journal of Sports Medicine and Physical Fitness, vol. 55, no. 1-2, pp. 58–67, 2015.
- L. Dalle Carbonare, M. Manfredi, G. Caviglia et al., “Can half-marathon affect overall health? The yin-yang of sport,” Journal of Proteomics, vol. 170, pp. 80–87, 2018.
- G. M. Niemiro, J. Parel, J. Beals et al., “Kinetics of circulating progenitor cell mobilization during submaximal exercise,” Journal of Applied Physiology, vol. 122, no. 3, pp. 675–682, 2017.
- P. Anversa, J. Kajstura, and A. Leri, “Circulating progenitor cells: search for an identity,” Circulation, vol. 110, no. 20, pp. 3158–3160, 2004.
- M. T. Valenti, L. D. Carbonare, L. Donatelli, F. Bertoldo, M. Zanatta, and V. Lo Cascio, “Gene expression analysis in osteoblastic differentiation from peripheral blood mesenchymal stem cells,” Bone, vol. 43, no. 6, pp. 1084–1092, 2008.
- M. T. Valenti, M. Mottes, S. Cheri et al., “Runx2 overexpression compromises bone quality in acromegalic patients,” Endocrine-Related Cancer, vol. 25, no. 3, pp. 269–277, 2018.
- L. Dalle Carbonare, M. Mottes, G. Malerba et al., “Enhanced osteogenic differentiation in zoledronate-treated osteoporotic patients,” International Journal of Molecular Sciences, vol. 18, no. 6, article 1261, 2017.
- L. D. Carbonare, M. T. Valenti, M. Zanatta, L. Donatelli, and V. Lo Cascio, “Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis,” Arthritis and Rheumatism, vol. 60, no. 11, pp. 3356–3365, 2009.
- M. T. Valenti, M. Mottes, A. Biotti et al., “Clodronate as a therapeutic strategy against osteoarthritis,” International Journal of Molecular Sciences, vol. 18, no. 12, p. 2696, 2017.
- I. R. Indran, M. P. Hande, and S. Pervaiz, “hTERT overexpression alleviates intracellular ROS production, improves mitochondrial function, and inhibits ROS-mediated apoptosis in cancer cells,” Cancer Research, vol. 71, no. 1, pp. 266–276, 2011.
- M. Mattiussi, G. Tilman, S. Lenglez, and A. Decottignies, “Human telomerase represses ROS-dependent cellular responses to tumor necrosis factor-α without affecting NF-κB activation,” Cellular Signalling, vol. 24, no. 3, pp. 708–717, 2012.
- R. Kiffin, U. Bandyopadhyay, and A. M. Cuervo, “Oxidative stress and autophagy,” Antioxidants & Redox Signaling, vol. 8, no. 1-2, pp. 152–162, 2006.
- F. Pucci, L. Gardano, and L. Harrington, “Short telomeres in ESCs lead to unstable differentiation,” Cell Stem Cell, vol. 12, no. 4, pp. 479–486, 2013.
- F. V. Sbrana, M. Cortini, S. Avnet et al., “The role of autophagy in the maintenance of stemness and differentiation of mesenchymal stem cells,” Stem Cell Reviews, vol. 12, no. 6, pp. 621–633, 2016.
- R. Emmons, G. M. Niemiro, O. Owolabi, and M. De Lisio, “Acute exercise mobilizes hematopoietic stem and progenitor cells and alters the mesenchymal stromal cell secretome,” Journal of Applied Physiology, vol. 120, no. 6, pp. 624–632, 2016.
- G. M. Niemiro, T. Edwards, J. P. Barfield et al., “Circulating progenitor cell response to exercise in wheelchair racing athletes,” Medicine & Science in Sports & Exercise, vol. 50, no. 1, pp. 88–97, 2018.
- K. Marycz, K. Mierzejewska, A. Smieszek et al., “Endurance exercise mobilizes developmentally early stem cells into peripheral blood and increases their number in bone marrow: implications for tissue regeneration,” Stem Cells International, vol. 2016, Article ID 5756901, 10 pages, 2016.
- M. Marędziak, A. Śmieszek, K. Chrząstek, K. Basinska, and K. Marycz, “Physical activity increases the total number of bone-marrow-derived mesenchymal stem cells, enhances their osteogenic potential, and inhibits their adipogenic properties,” Stem Cells International, vol. 2015, Article ID 379093, 11 pages, 2015.
- G. M. Niemiro, J. M. Allen, L. J. Mailing et al., “Effects of endurance exercise training on inflammatory circulating progenitor cell content in lean and obese adults,” The Journal of Physiology, vol. 596, no. 14, pp. 2811–2822, 2018.
- M. R. Bonsignore, G. Morici, A. Santoro et al., “Circulating hematopoietic progenitor cells in runners,” Journal of Applied Physiology, vol. 93, no. 5, pp. 1691–1697, 2002.
- X. Wu, Z. Wang, M. Qian, L. Wang, C. Bai, and X. Wang, “Adrenaline stimulates the proliferation and migration of mesenchymal stem cells towards the LPS-induced lung injury,” Journal of Cellular and Molecular Medicine, vol. 18, no. 8, pp. 1612–1622, 2014.
- C. Lopez-Otin, M. A. Blasco, L. Partridge, M. Serrano, and G. Kroemer, “The hallmarks of aging,” Cell, vol. 153, no. 6, pp. 1194–1217, 2013.
- A. G. Bodnar, M. Ouellette, M. Frolkis et al., “Extension of life-span by introduction of telomerase into normal human cells,” Science, vol. 279, no. 5349, pp. 349–352, 1998.
- A. T. Ludlow, E. E. Spangenburg, E. R. Chin, W. H. Cheng, and S. M. Roth, “Telomeres shorten in response to oxidative stress in mouse skeletal muscle fibers,” The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, vol. 69, no. 7, pp. 821–830, 2014.
- N. Hariharan and M. A. Sussman, “Cardiac aging - getting to the stem of the problem,” Journal of Molecular and Cellular Cardiology, vol. 83, pp. 32–36, 2015.
- E. Fathi, H. N. Charoudeh, Z. Sanaat, and R. Farahzadi, “Telomere shortening as a hallmark of stem cell senescence,” Stem Cell Investigation, vol. 6, p. 7, 2019.
- E. Hiyama and K. Hiyama, “Telomere and telomerase in stem cells,” British Journal of Cancer, vol. 96, no. 7, pp. 1020–1024, 2007.
- B. M. Abdallah, M. Haack-Sorensen, J. S. Burns et al., “Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene despite of extensive proliferation,” Biochemical and Biophysical Research Communications, vol. 326, no. 3, pp. 527–538, 2005.
- J. L. Simonsen, C. Rosada, N. Serakinci et al., “Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells,” Nature Biotechnology, vol. 20, no. 6, pp. 592–596, 2002.
- A. Shirakabe, Y. Ikeda, S. Sciarretta, D. K. Zablocki, and J. Sadoshima, “Aging and autophagy in the heart,” Circulation Research, vol. 118, no. 10, pp. 1563–1576, 2016.
- Z. Zhou, G. Shi, X. Zheng, S. Jiang, and L. Jiang, “Autophagy activation facilitates mechanical stimulation-promoted osteoblast differentiation and ameliorates hindlimb unloading-induced bone loss,” Biochemical and Biophysical Research Communications, vol. 498, no. 3, pp. 667–673, 2018.
- Y. Ma, M. Qi, Y. An et al., “Autophagy controls mesenchymal stem cell properties and senescence during bone aging,” Aging Cell, vol. 17, no. 1, 2018.
- R. Singh, Y. Xiang, Y. Wang et al., “Autophagy regulates adipose mass and differentiation in mice,” The Journal of Clinical Investigation, vol. 119, no. 11, pp. 3329–3339, 2009.
- K. Marycz, K. Kornicka, K. Basinska, and A. Czyrek, “Equine metabolic syndrome affects viability, senescence, and stress factors of equine adipose-derived mesenchymal stromal stem cells: new insight into EqASCs isolated from EMS horses in the context of their aging,” Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 4710326, 17 pages, 2016.
- K. Marycz, K. Kornicka, J. Grzesiak, A. Smieszek, and J. Szlapka, “Macroautophagy and selective mitophagy ameliorate chondrogenic differentiation potential in adipose stem cells of equine metabolic syndrome: new findings in the field of progenitor cells differentiation,” Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 3718468, 18 pages, 2016.
- K. Marycz, K. Kornicka, J. Szlapka-Kosarzewska, and C. Weiss, “Excessive endoplasmic reticulum stress correlates with impaired mitochondrial dynamics, mitophagy and apoptosis, in liver and adipose tissue, but not in muscles in EMS horses,” International Journal of Molecular Sciences, vol. 19, no. 1, p. 165, 2018.
- M. Markaki and N. Tavernarakis, “Metabolic control by target of rapamycin and autophagy during ageing - a mini-review,” Gerontology, vol. 59, no. 4, pp. 340–348, 2013.
- J. Roh, Y. Kim, J. Oh et al., “Hexokinase 2 is a molecular bridge linking telomerase and autophagy,” PLoS One, vol. 13, no. 2, article e0193182, 2018.
- H. Cheng, X. Fan, W. E. Lawson, P. Paueksakon, and R. C. Harris, “Telomerase deficiency delays renal recovery in mice after ischemia-reperfusion injury by impairing autophagy,” Kidney International, vol. 88, no. 1, pp. 85–94, 2015.
- N. Makino, J. Oyama, T. Maeda, M. Koyanagi, Y. Higuchi, and K. Tsuchida, “Calorie restriction increases telomerase activity, enhances autophagy, and improves diastolic dysfunction in diabetic rat hearts,” Molecular and Cellular Biochemistry, vol. 403, no. 1-2, pp. 1–11, 2015.
- V. H. Heyward, Ed.“The Physical Fitness Specialist Certification Manual,” in revised 1997 printed in Advance Fitness Assessment & Exercise Prescription, p. 48, The Cooper Institute for Aerobics Research, Dallas TX, USA, 3rd edition, 1998.
- D. Rapp, J. Scharhag, S. Wagenpfeil, and J. Scholl, “Reference values for peak oxygen uptake: cross-sectional analysis of cycle ergometry-based cardiopulmonary exercise tests of 10 090 adult German volunteers from the Prevention First Registry,” BMJ Open, vol. 8, no. 3, article e018697, 2018.
- M. Barrios Vergara, J. Ocaranza Ozimica, L. Llach Fernández, C. Osorio Fuentealba, V. Giner Costagliola, and C. Sacomori, “VO2 máximo indirecto y edad fitness de sedentarios y no sedentarios // VO2 indirect maximum and fitness age of sedentary and non-sedentary,” Revista Internacional de Medicina y Ciencias de la Actividad Física y del Deporte, vol. 18, no. 71, pp. 493–505, 2018.
- S. J. Thoma, C. P. Lamping, and B. L. Ziegler, “Phenotype analysis of hematopoietic CD34+ cell populations derived from human umbilical cord blood using flow cytometry and cDNA-polymerase chain reaction,” Blood, vol. 83, no. 8, pp. 2103–2114, 1994.
- B. Klotz, B. Mentrup, M. Regensburger et al., “1,25-Dihydroxyvitamin D3 treatment delays cellular aging in human mesenchymal stem cells while maintaining their multipotent capacity,” PLoS One, vol. 7, no. 1, article e29959, 2012.
- L. Ferroni, I. Tocco, A. De Pieri et al., “Pulsed magnetic therapy increases osteogenic differentiation of mesenchymal stem cells only if they are pre-committed,” Life Sciences, vol. 152, pp. 44–51, 2016.
- M. T. Valenti, U. Garbin, A. Pasini et al., “Role of Ox-PAPCs in the differentiation of mesenchymal stem cells (MSCs) and Runx2 and PPARγ 2 expression in MSCs-like of osteoporotic patients,” PLoS One, vol. 6, no. 6, article e20363, 2011.
- A. L. Buchman, C. Keen, J. Commisso et al., “The effect of a marathon run on plasma and urine mineral and metal concentrations,” Journal of the American College of Nutrition, vol. 17, no. 2, pp. 124–127, 1998.
- L. A. Cohen, L. Gutierrez, A. Weiss et al., “Serum ferritin is derived primarily from macrophages through a nonclassical secretory pathway,” Blood, vol. 116, no. 9, pp. 1574–1584, 2010.
- M. Truman-Rosentsvit, D. Berenbaum, L. Spektor et al., “Ferritin is secreted via 2 distinct nonclassical vesicular pathways,” Blood, vol. 131, no. 3, pp. 342–352, 2018.
- F. Seifi-Skishahr, A. Damirchi, M. Farjaminezhad, and P. Babaei, “Physical training status determines oxidative stress and redox changes in response to an acute aerobic exercise,” Biochemistry Research International, vol. 2016, Article ID 3757623, 9 pages, 2016.
- S. Krishan, P. J. Jansson, E. Gutierrez, D. J. Lane, D. Richardson, and S. Sahni, “Iron metabolism and autophagy: a poorly explored relationship that has important consequences for health and disease,” Nagoya Journal of Medical Science, vol. 77, no. 1-2, pp. 1–6, 2015.
- T. A. Zachos, K. M. Shields, and A. L. Bertone, “Gene-mediated osteogenic differentiation of stem cells by bone morphogenetic proteins-2 or -6,” Journal of Orthopaedic Research, vol. 24, no. 6, pp. 1279–1291, 2006.
- S. Modica and C. Wolfrum, “The dual role of BMP4 in adipogenesis and metabolism,” Adipocytes, vol. 6, no. 2, pp. 141–146, 2017.
- K. Miyazono, Y. Kamiya, and M. Morikawa, “Bone morphogenetic protein receptors and signal transduction,” Journal of Biochemistry, vol. 147, no. 1, pp. 35–51, 2010.
- S. Modica, L. G. Straub, M. Balaz et al., “Bmp4 promotes a brown to white-like adipocyte shift,” Cell Reports, vol. 16, no. 8, pp. 2243–2258, 2016.
- A. Leonardini, L. Laviola, S. Perrini, A. Natalicchio, and F. Giorgino, “Cross-talk between PPARγ and insulin signaling and modulation of insulin sensitivity,” PPAR Research, vol. 2009, Article ID 818945, 12 pages, 2009.
- M. T. Valenti, M. Deiana, S. Cheri et al., “Physical exercise modulates miR-21-5p, miR-129-5p, miR-378-5p, and miR-188-5p expression in progenitor cells promoting osteogenesis,” Cells, vol. 8, no. 7, p. 742, 2019.
- M. Cernea, W. Tang, H. Guan, and K. Yang, “Wisp1 mediates Bmp3-stimulated mesenchymal stem cell proliferation,” Journal of Molecular Endocrinology, vol. 56, no. 1, pp. 39–46, 2016.
- L. Liu, C. M. DiGirolamo, P. A. A. S. Navarro, M. A. Blasco, and D. L. Keefe, “Telomerase deficiency impairs differentiation of mesenchymal stem cells,” Experimental Cell Research, vol. 294, no. 1, pp. 1–8, 2004.
- N. Serakinci, J. Graakjaer, and S. Kolvraa, “Telomere stability and telomerase in mesenchymal stem cells,” Biochimie, vol. 90, no. 1, pp. 33–40, 2008.
- S. Ahmed, J. F. Passos, M. J. Birket et al., “Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress,” Journal of Cell Science, vol. 121, no. 7, pp. 1046–1053, 2008.
- Y. Wan, N. Zhuo, Y. Li, W. Zhao, and D. Jiang, “Autophagy promotes osteogenic differentiation of human bone marrow mesenchymal stem cell derived from osteoporotic vertebrae,” Biochemical and Biophysical Research Communications, vol. 488, no. 1, pp. 46–52, 2017.
- T. Torisu, K. Torisu, I. H. Lee et al., “Autophagy regulates endothelial cell processing, maturation and secretion of von Willebrand factor,” Nature Medicine, vol. 19, no. 10, pp. 1281–1287, 2013.
- D. Zhang, B. Tang, X. Xie, Y. F. Xiao, S. M. Yang, and J. W. Zhang, “The interplay between DNA repair and autophagy in cancer therapy,” Cancer Biology & Therapy, vol. 16, no. 7, pp. 1005–1013, 2015.
- K. Czornak, S. Chughtai, and K. H. Chrzanowska, “Mystery of DNA repair: the role of the MRN complex and ATM kinase in DNA damage repair,” Journal of Applied Genetics, vol. 49, no. 4, pp. 383–396, 2008.
- P. K. Mandal, C. Blanpain, and D. J. Rossi, “DNA damage response in adult stem cells: pathways and consequences,” Nature Reviews Molecular Cell Biology, vol. 12, no. 3, pp. 198–202, 2011.
- C. Blanpain, M. Mohrin, P. A. Sotiropoulou, and E. Passegue, “DNA-damage response in tissue-specific and cancer stem cells,” Cell Stem Cell, vol. 8, no. 1, pp. 16–29, 2011.
- L. Oliver, E. Hue, Q. Sery et al., “Differentiation-related response to DNA breaks in human mesenchymal stem cells,” Stem Cells, vol. 31, no. 4, pp. 800–807, 2013.
- J. Jakovljevic, C. R. Harrell, C. Fellabaum, A. Arsenijevic, N. Jovicic, and V. Volarevic, “Modulation of autophagy as new approach in mesenchymal stem cell-based therapy,” Biomedicine & Pharmacotherapy, vol. 104, pp. 404–410, 2018.
Copyright © 2019 L. Dalle Carbonare et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.