KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2/ARE Signaling In Vitro and In Vivo

Figure 2

Expression of KLF2 in SW1353 cells stimulated with IL-1β at different concentrations for various durations. (a, b) SW1353 cells were pretreated with IL-1β (20 ng/ml) for the indicated times or with IL-1β (4 h) at the indicated concentration. KLF2 expression was measured by real-time PCR (a) and Western blot analysis (b). β-Actin was used as an endogenous control. Quantitative analysis of KLF2 protein levels based on the specific signal intensities measured using ImageJ. (c–e) Detection of KLF2, MMP13, and COL2A1 expression in SW1353 cells treated with IL-1β (20 ng/ml, 224) by immunofluorescence microscopy. The scale bar represents 50 μm. All data are expressed as the . , , and .

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