Oleuropein-Induced Apoptosis Is Mediated by Mitochondrial Glyoxalase 2 in NSCLC A549 Cells: A Mechanistic Inside and a Possible Novel Nonenzymatic Role for an Ancient Enzyme

Figure 3

OP drives apoptosis in NSCLC A549 cells by promoting mGlo2 association to Bax. (a) Mitochondrial lysates from A549 cells treated with 150 μM oleuropein (OP) were immunoprecipitated with protein G agarose-coupled anti-Glo2 (IP : Glo2) and subjected to western blotting (WB) with the anti-Bax antibody (WB : anti-Bax). Blots were then stripped and reprobed with the anti-Glo2 antibody to ensure equal immunoprecipitation of Glo2 protein. Mouse IgG was used as a negative control for immunoprecipitation; (b) mitochondrial lysates from A549 cells treated with 150 μM OP under SOD2 silencing (siSOD2) or control (siCtr, nonspecific siRNA) were immunoprecipitated with protein G agarose-coupled anti-Glo2 (IP : Glo2) and subjected to western blotting (WB) with the anti-Bax antibody (WB : anti-Bax).

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