Overexpression of BRCA1 in Neural Stem Cells Enhances Cell Survival and Functional Recovery after Transplantation into Experimental Ischemic Stroke
Overexpression of BRCA1 reduced NSCs apoptosis after OGD/R. NSCs were transfected with LV-GFP or LV-BRCA1 before OGD administration. (a) TUNEL-AP staining and PI/Hoechst 333342 double staining were performed to detect cell apoptosis 24 h after reoxygenation. (b) Quantification of PI-positive NSCs. OGD/R administration increased apoptosis of NSCs, which was attenuated by LV-BRCA1 transfection. Data are expressed as ;. vs. the control group; vs. the OGD/R group. Scale bar: 50 μm.