miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling

Figure 2

miR-154-5p induces cardiac hypertrophy and apoptosis by promoting oxidative stress and inflammatory reactions in cardiomyocytes. (a) Images of the cell area labeled with Actin-Tracker Green (). (b) Results from the real-time PCR analysis of the expression of the ANF, BNP, and β-MHC mRNAs in cardiomyocytes transfected with normal control scrambled RNA (NC) and miR-154-5p mimics (). (c) Images of TUNEL staining and quantification of the percentage of apoptotic cells (). (d) Western blots and the quantification of cleaved caspase-3 and caspase-3 levels (). (e) Images of the cell area labeled with Actin-Tracker Green staining and quantitative analysis of the control, AngII, normal control inhibitor RNA with AngII (NCi+AngII), and miR-154 inhibitor with AngII (miR-154i+AngII) groups (). (f) Images and quantitative analysis of TUNEL staining (). (g) DHE staining was used to analyze ROS levels (). (h) Images of double immunofluorescence staining showing the NF-κB (green) localization. (i) Western blot and quantitative analysis of caspase-3 and cleaved caspase-3 levels (). (j) Average levels of ANF, BNP, and β-MHC mRNA expression (). , , , and .

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