miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling

Figure 4

Upregulation of Arsb reverses the effects of miR-154-5p on cardiomyocyte hypertrophy and apoptosis. (a) Expression of markers of cardiac hypertrophy in cardiomyocytes transfected with scramble, si-Arsb, as measured using real-time PCR (). (b) Western blot images showing the levels of Arsb, cleaved caspase-3, and caspase-3 in cardiomyocytes subjected to different treatments (). (c) Expression of markers of cardiac hypertrophy in cardiomyocytes transfected with NC, miR-154-5p, miR-154-5p+vector, and miR-154-5p+Arsb, as measured using real-time PCR (). (d) Western blot images showing the levels of Arsb, cleaved caspase-3, and caspase-3 in cardiomyocytes subjected to different treatments (). (e, f) Western blot images showing the levels of the p-JNK, JNK, p-p38, p38, p-STAT3, and STAT3 proteins in the Con, AngII, AngII+vector, and AngII+Arsb groups () and in the NC, miR-154-p, miR-154-5p+vector, and miR-154-5p+Arsb groups ().

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