Metformin Protects against Oxidative Stress Injury Induced by Ischemia/Reperfusion via Regulation of the lncRNA-H19/miR-148a-3p/Rock2 Axis

Figure 7

Inhibition of lncRNA-H19 expression influenced the effect of metformin on oxidative stress following OGD/R in vitro via regulation of Rock2/HO-1/Nrf2. (a) Activities of SOD in the supernatant of cell culture medium. (b) Levels of MDA in the supernatant of cell culture medium. (c, d) The protein expressions of Rock2, eNOS, HO-1, and Nrf2 were determined after transfection of pcDNA3.1-NC and pcDNA3.1-H19. (e) The mRNA expressions of Rock2, eNOS, HO-1, and Nrf2 were determined after transfection of pcDNA3.1-NC and pcDNA3.1-H19. Data are represented as . and versus the normal group by 1-way analysis of variance with Tukey’s multiple comparison test. ^#, ^##, and ^### versus the OGD/R group by 1-way analysis of variance with Tukey’s multiple comparison test. ^&, ^&&, and ^&&& versus the OGD/R+Met+pcDNA3.1-NC group by 1-way analysis of variance with Tukey’s multiple comparison test.

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