Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans

Figure 4

Effect of AO extracts on the expression of stress resistance-related genes and nuclear localization of DAF-16. (a) AO extracts decreased HSP 16.2 expression in mutant TJ375 worms (HSP-16.2::GFP(gplsI)) under oxidative stress induced by juglone. (b) AO extracts increased SOD-3 expression in mutant CF1553 worms ((pAD76) sod-3p::GFP+rol-6), and (c) AO extracts induced a significant translocation of DAF-16::GFP in mutant TJ356 worms (daf-16p::daf-16a/b::GFP+rol-6). (ci) Representative fluorescent images of the subcellular location of DAF-16 in the nucleus, intermediate, and cytosolic regions. (ai–aix) and (bi–bix) Representative pictures of GFP fluorescence in worms treated with 25 μg/mL AOH (ai/bi), 50 μg/mL AOH aii/bii), 100 μg/mL AOH (aiii/biii), 1 μg/mL AOM (aiv/biv), 2.5 μg/mL AOM (av/bv), 5 μg/mL AOM (avi/bvi), untreated control (avii/bvii), DMSO solvent control (aviii/bviii), and 25 μg/mL EGCG (aix/bix). The GFP mean pixel density for each group was calculated from the mean value of the 30 worms that were randomly selected. Data were obtained from three independent experiments and are presented as the . DMSO and EGCG were used as the solvent control group and positive control group, respectively. ,, , and , compared to the DMSO control; compared to the EGCG-positive control by one-way ANOVA following Bonferroni’s method (post hoc).

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