Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans
Effect of AO extracts on the expression of stress resistance genes and nuclear localization of SKN-1. (a) AO extract treatment increased GST-4 expression in mutant CL2166 worms ((pAF15)GST-4p::GFP::NLS) under oxidative stress induced by juglone. (b) AO extracts treatment induced a significant translocation of SKN-1::GFP in mutant LD1 worms. (bi) Representative fluorescent images of the subcellular location of SKN-1 in the nucleus and cytosol. (ai–aix) Representative pictures of GFP fluorescence in worms treated with 25 μg/mL AOH (ai), 50 μg/mL AOH (aii), 100 μg/mL AOH (aiii), 1 μg/mL AOM (aiv), 2.5 μg/mL AOM (av), 5 μg/mL AOM (avi), untreated control (avii), DMSO solvent control (aviii), and 25 μg/mL EGCG (aix). The GFP mean pixel density for each group was calculated from the mean value of the 30 worms that were randomly selected. Data were obtained from three independent experiments and presented as the . DMSO and EGCG were used as the solvent control group and positive control group, respectively. ,,, and , compared to the DMSO control by one-way ANOVA following Bonferroni’s method (post hoc).
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