Research Article

Dihydrotanshinone-Induced NOX5 Activation Inhibits Breast Cancer Stem Cell through the ROS/Stat3 Signaling Pathway

Figure 2

The effect of DHTS on tumor growth in a xenograft model. Three million cells were injected into the mammary fat pad of each immunodeficient NOD-SCID female nude mouse. Analysis of the effect of tumor growth on DHTS and MCF-7 cell-bearing immunodeficient nude mice. The dose of drug used was 10 mg/kg once a week. After 13 weeks, images were captured with an Odyssey® Imager (LICOR, Pearl Image System, USA). The weights of mice were comparable with the control and DHTS-treated groups (a). The high grade of the tumors was detected using the IRDye 800CW optical probe (2DG) in the 800 nm channel, represented in pseudo-color. Tumor volume was measured twice/week using a caliper and calculated as . Tumor growth curves were monitored during the experimental period (b). The effect of DHTS on tumor weights. Tumor weights were measured after therapy. compared to the control (c). Representative images were captured at the end of 13 weeks of therapy, and the results are shown for vehicle-treated control and DHTS-treated mice.
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