Research Article
Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress
Figure 2
Blocking α2A-AR siRNA reversed the protective role of Dex on OLT-induced intestinal mucosal barrier injury in rats. (a–b) Western blotting results showing the levels of occludin (a) and ZO-1 (b) in rat intestines. (c–f) ELISA results showing the levels of serum biomarkers of intestinal mucosal barrier function, including DAO (c), LPS (d), I-FABP2 (e), and D-LA (f). Data are represented as the , for each group. S: sham-operated group; M: the model group with OLT; D1: rats that were pretreated with 10 μg/kg before OLT; D2: rats that were pretreated with 50 μg/kg before OLT; B1: rats that received 500 g/kg atipamezole at 40 min before receiving 50 μg/kg Dex prior to OLT; B2: rats that received 50 g/kg ARC239 at 40 min before receiving 50 μg/kg Dex prior to OLT; B3: rats that received 1.5 mg/kg BRL-44408 at 40 min before receiving 50 μg/kg Dex prior to OLT. , compared to Group S; #, compared to Group M; $, compared to Group D2.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |
| (f) |