Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α₂-Adrenergic Receptor-Dependent Suppression of Oxidative Stress

Figure 4

Silencing of α_2A-AR siRNA reversed the protective role of Dex on cell apoptosis in IEC-6 cells with simulated H/R stimulation. (a) qRT-PCR data showing effective knockdown of α_2A-AR by siRNA transfection in IEC-6 cells. Control: untreated IEC-6 cells; NC-siRNA: IEC-6 cells transfected with nonspecific control siRNA; α_2A-AR siRNA: IEC-6 cells transfected with α_2A-AR-specific siRNA. (b) Results of the cell proliferation assay by Cell Counting Kit-8 in IEC-6 cells with different stimulations. (c) Summary of cell apoptosis data of IEC-6 cells with different stimulations. (d) Representative flow cytometry profiles showing the cell apoptosis assay by annexin V and propidium iodide staining, for each group. A: control IEC-6 cells; B: IEC-6 cells with H/R treatment; C: IEC-6 cells that were pretreated with 1 nM Dex for 1 h before inducing H/R injury; D: IEC-6 cells with silencing of α_2A-AR siRNA; E: IEC-6 cells with silencing of α_2A-AR siRNA and H/R treatment; F: IEC-6 cells with silencing of α_2A-AR siRNA that were pretreated with Dex prior to H/R injury. , compared to Group A; ^#, compared to Group B; ^$, compared to Group C.

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